猕猴桃根响应轮纹镰孢菌侵染的转录组分析OA

[Objective]To understand the physiological and biochemical changes and molecular response characteristics of kiwifruit roots infected by root rot pathogen.[Method]Kiwifruit'Hongyang'roots were inoculated with Fusarium concentricum,the causal agent of kiwifruit root rot.Changes in soluble sugar content and activities of antioxidant enzymes such as peroxidase in roots were analyzed after inoculation.Meanwhile,transcriptome sequencing was used to identify the key pathways and differentially expressed genes(DEGs)in kiwifruit roots in response to Fusarium concentricum infection.[Result]Fusarium concentricum infection increased the activities of POD in kiwifruit roots,but decreased the activities of SOD and CAT.Transcriptome sequencing revealed 12 267,14 878 and 11 864 DEGs at 6,12 and 24 h post inoculation,respectively.These DEGs were mainly associated with cell components including photosystem and membrane,exerted molecular functions such as protein serine/threonine kinase activity and oxidoreductase activity,and participated in biological processes including photosynthesis and response to wounding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that the plant-pathogen interaction pathway was the major metabolic pathway in kiwifruit roots responding to Fusarium concentricum infection.The number of DEGs enriched in this pathway ranged from 406 to 479,mainly involving plasma membrane-localized calcium-transporting genes CNGCs,and cytoplasmic CDPK and Rboh genes related to reactive oxygen species(ROS)production.[Conclusion]These results lay a foundation for dissecting the physiological and molecular mechanisms of kiwifruit roots in response to Fusarium concentricum infection,and provide candidate gene resources for resistance breeding of kiwifruit root rot.