一株黄颡鱼源鮰爱德华菌的药敏特性及全基因组测序分析OA
Antimicrobial susceptibility and whole-genome sequencing and analysis of an Edwardsiella ictaluri strain YCH from yellow catfish
鮰爱德华菌(Edwardsiella ictaluri)是水产养殖中重要的病原菌之一,近年来其耐药性及跨种传播风险不断上升,而不同宿主来源菌株的基因组研究对于揭示其致病机制、流行规律、遗传多样性与适应性具有重要意义.为解析一株黄颡鱼源鮰爱德华菌YCH的耐药特性及基因组特征和进化关系,对其开展药敏试验和全基因组测序组装,结合多数据库进行功能注释,并与 9 株爱德华菌属菌株开展比较与泛基因组分析.结果显示:爱德华菌YCH基因组大小为3.79 Mb,GC含量为57.42%,包含3 439 个编码基因、25 个rRNA和99 个tRNA,共检测到 34 个基因岛和 6 个前噬菌体,未检测到CRISPR-Cas位点.药敏试验和耐药基因分析表明,该菌株可能主要通过外排泵和靶点修饰机制对红霉素、四环素和氟喹诺酮类药物等呈现多重耐药性.功能注释显示该菌株的代谢通路基因丰富,各数据库分别注释基因 3 564(GO)、3 078(COG)和3 384(KEGG)个.泛基因组学分析表明,YCH与其他 9 株爱德华菌共享 2 294 个核心基因,而特有基因数量差异显著(29～547 个).系统发育分析进一步揭示,YCH与 爱德华菌Ei-VN5 聚类于同一进化分支.本研究首次报道黄颡鱼源鮰爱德华菌YCH的全基因组测序及功能注释,系统揭示其基因组特征、功能分布、耐药性与进化关系,可为深入解析 爱德华菌的致病和耐药机制及制定防控策略提供重要参考.
Edwardsiella ictaluri is an important pathogen in aquaculture.In recent years,its increasing antibiotic resistance and cross-species transmission have raised serious concerns.Genomic studies of strains from different hosts are essential for exploring their pathogenic mechanisms,epidemiology,genetic diversity,and adaptive e-volution.To elucidate the antimicrobial resistance,genomic characteristics,and evolutionary relationships of an E.ictaluri strain YCH from yellow catfish,antimicrobial susceptibility testing and whole-genome sequencing and assembly were performed.Functional annotation was performed using multiple databases,and comparative and pan-genome analyses were conducted with nine other Edwardsiella strains.The results showed that the YCH ge-nome is 3.79 Mb in size,with a GC content of 57.42%,and comprises 3 439 coding sequences,25 rRNAs,and 99 tRNAs.A total of 34 genomic islands and 6 prophages were identified,while no CRISPR-Cas loci were detected.Antimicrobial susceptibility testing and resistance gene prediction indicated that the YCH strain might employ efflux pumps and target modification mechanisms to exhibit multidrug resistance to polymyxins,erythro-mycin,tetracyclines,and fluoroquinolones.Functional annotation revealed a large number of genes involved in metabolic pathways,with 3 564,3 078,and 3 384 genes assigned to GO,COG,and KEGG databases,respectively.Pan genomic analysis showed that YCH shares 2 294 conserved core genes with nine other Ed-wardsiella strains,while the number of strain-specific genes varied significantly(29～547).Phylogenetic analysis further revealed that YCH clusters closely with E.ictaluri Ei-VN5 in the same evolutionary branch.This study represents the first report of whole-genome sequencing and functional annotation of E.ictaluri strain YCH from yellow catfish,systematically revealing its genomic characteristics,functional distribution,antibiotic resistance,and evolutionary relationships.These findings provide an important insights into the pathogenicity and resistance mechanisms of E.ictaluri and offer valuable references for developing prevention and control strategies.
孔乐;郭锦志;龚建森;魏文志;刘晓丹;付立霞
扬州大学动物科学与技术学院,江苏 扬州 225009扬州大学动物科学与技术学院,江苏 扬州 225009中国农业科学院家禽研究所,江苏 扬州 225125扬州大学动物科学与技术学院,江苏 扬州 225009扬州大学动物科学与技术学院,江苏 扬州 225009扬州大学动物科学与技术学院,江苏 扬州 225009
农业科技
鮰爱德华菌(Edwardsiella ictaluri)全基因组测序比较基因组耐药性毒力因子
Edwardsiella ictaluriwhole-genome sequencingcomparative genomicsantibiotic resistanceviru-lence factors
《淡水渔业》 2026 (2)
68-78,11
江苏省农业科技自主创新资金项目(CX(24)3066)农业农村部渔用药物创制重点实验室开放课题(201801)
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