舞毒蛾CTL基因家族鉴定及对抗真菌能力的影响OA

To investigate the effects of the C-type lectin(CTL)gene family on the resistance of Lymantria dispar to Beauveria bassiana,a comprehensive bioinformatic analysis and identification of the L.dispar CTL family were performed.The results showed that most CTL contained two CTLD conserved domains.Among them,the L.dispar CTL-10 protein exhibited a close phylogenetic relationship with the previously identified CTLs from Lepidoptera species.After infection with B.bassi-ana,the expression levels of CTL genes in L.dispar were significantly up-regulated within 48 hours.Furthermore,after in-hibiting CTL-10 for 96 hours,it resulted in a 26%increase in the mortality rate of L.dispar caused by B.bassiana.The cellular immune capacity of L.dispar was further detected.The knockdown of CTL-10 significantly reduced the total hemo-cyte count and encapsulation ability of L.dispar.In addition,B.bassiana infection could induce the expression of genes in the Toll signaling pathway of L.dispar,while the knockdown of CTL-10 significantly attenuated this inductive effect of B.bassiana on the aforementioned genes.Collectively,these results demonstrated that CTL-10 is a crucial gene involved in the resistance of L.dispar to B.bassiana invasion.The analysis of the antifungal activity of CTL-10 will facilitate the iden-tification of novel targets for the green biological control of L.dispar.