依据表型指标和分子标记的红松种子园遗传多样性分析OA

To clarify the genetic background of Pinus koraiensis seed orchard,24 clones from the first-generation clonal seed or-chard of P.koraiensis at the Experimental Forest Farm of Liaoning Provincial Institute of Forest Management were selected as research materials.We analyzed the differences and diversity of 13 phenotypic traits among P.koraiensis clones,and evaluated the genetic diversity of the seed orchard at the molecular level using SSR marker technology,thereby constructing molecular identity cards for each P.koraiensis clone.The results showed that the P.koraiensis seed orchard exhibited abun-dant genetic diversity at both phenotypic and molecular levels.The coefficient of variation(CV)of the 13 phenotypic traits ranged from 7.48%to 91.77%,with an average of 23.67%.Among them,the mean CV values of growth traits and seed traits were 18.90%and 31.30%,respectively.The Shannon-Weaver diversity index varied from 0.928 to 2.084,with an average of 1.908,and the mean diversity index values of growth traits and seed traits were 1.890 and 1.938,respectively.These findings indicated that the growth traits of different clones in the seed orchard showed large numerical differences,while the seed traits had a more abundant numerical classification.In terms of molecular marker results,a total of 43 al-leles were detected in the 24 P.koraiensis clones using 10 pairs of SSR primers.The mean values of the effective number of alleles,Shannon's information index,expected heterozygosity,and observed heterozygosity were 2.207,0.906,0.476,and 0.421,respectively.Cluster analysis based on both phenotypic traits and SSR markers divided the clones in the seed orchard into three major groups,but there were significant differences in clone numbers within each group.Furthermore,molecular identity cards for the 24 P.koraiensis clones were constructed using 3 pairs of high-efficiency primers,with a resolution rate of 100%.