有机长余辉纳米探针介导的LFIA胃蛋白酶原检测OA

Ultra-long organic phosphorescent materials have the advantages of rich excited state properties,low price and easy to synthesize,good flexibility,ultra-long luminescence lifetime.After the fluorescence decay of the background,the collection of the remaining light can effectively eliminate the background light interference.Based on this,a Lateral Flow Immunochromatographic Detection Platform Based on Ultra-Long Organo-Phosphorescent Nanoprobes(UOP-LFIAs)constructed by authors is presented in this paper.Firstly,long afterglow crystals were prepared and synthesized,and then the long afterglow solution was obtained by one-step coating polyvinylpyrrolidone and carboxymethyl cellulose on the surface.Next,it was coupled with antibodies to obtain ultra-long organic afterglow phosphorescent nanoprobes.A portable UOP-LFIAs test strip detection analyzer was then designed and manufactured.Finally,Pepsinogen(PG)I and II were detected by using a portable UOP-LFIAs detection device.The results show that the detection limits were 0.36 ng/mL and 0.57 ng/mL for PG I and PG II,with respective detection ranges of 0.36-1 200 ng/mL and 0.57-500 ng/mL.The average recoveries were 101.30%and 99.30%,respectively.It is concluded that the UOP-LFIAs assay platform established in this study is not only simple,rapid,cost-effective and suitable for macro-preparation,but also can effectively reduce the interference of background light signal and enhance the phosphorescence efficiency,thereby improving the detection sensitivity.Additionally,the platform enables the combined detection of PG I and PG II,providing results in just 5 minutes.T his development offers a powerful tool for bedside disease detection.