荷叶多糖NFP-0.8-b的纯化、磷酸化修饰及其生物活性OA

Polysaccharides from Nelumbinis Folium(NFP)were extracted with deionized water as the solvent.A novel polysaccharide NFP-0.8-b was purified by DEAE-Sepharose Fast Flow anion exchange column and Sephadex G-100 gel chromatography column.On this basis,the optimal phosphorylation conditions for NFP-0.8-b were determined.The antioxidant activity of phosphorylated NFP-0.8-b(Ph-NFP-0.8-b)and its inhibitory effect on advanced glycation end products during cookie production were investigated.The results show that the optimal phosphorylation process of NFP-0.8-b is as follows:phosphorylation reagent ratio(sodium tripolyphosphate︰sodium trimetaphosphate)5︰2,reaction temperature 75℃,pH 9.0,and reaction time 3.5 h.Under these conditions,the phosphate content reaches 8.39%.Compared with NFP-0.8-b,the scavenging effect of Ph-NFP-0.8-b on DPPH free radicals and hydroxyl free radicals are further enhanced.When the polysaccharide content is greater than 5%,the inhibitory effect of Ph-NFP-0.8-b on advanced glycation end products during cookie production is significantly stronger than that of NFP-0.8-b(P＜0.05),suggesting that Ph-NFP-0.8-b could be used as an antioxidant and inhibitor of advanced glycation end products in the industrial production of cookies.