微波辅助低共熔溶剂提取海带多糖的工艺优化及抗氧化活性分析OA
Process optimization and antioxidant activity analysis of microwave-assisted deep eutectic solvent extraction of laminaria japonica polysaccharides
为探究海带多糖的最佳提取工艺,并对其结构特性及抗氧化活性进行分析,采用微波辅助低共熔溶剂(deep eutectic solvent,DES)提取海带多糖,通过单因素试验和响应面试验获得最佳提取工艺,利用傅里叶变换红外光谱、X-射线衍射和场发射扫描电子显微镜等对其进行结构表征,同时对其抗氧化活性进行分析.结果表明,海带多糖的最优提取工艺为含水质量分数20%的DES(甜菜碱与尿素摩尔比1∶2)、固液比1∶40(g/mL)、微波功率426 W、提取温度56℃、提取时间60 min,在此条件下获得多糖质量分数为5.96%,比热水浸提法提高52.04%;傅里叶变换红外光谱分析显示,微波辅助DES提取的海带多糖结构具有多样性;基于X-射线衍射分析,海带多糖为非结晶物质;场发射扫描电镜显示,热水浸提、微波辅助DES提取对海带多糖结构有不同影响.微波辅助甜菜碱-尿素提取的海带多糖具有较好的抗氧化活性,对ABTS+·自由基、DPPH自由基清除率分别为83.40%,62.34%.研究为开发和优化海带多糖的高值化利用提供理论依据.
To explore the optimal extraction process for laminaria japonica polysaccharides(LJP)and to analyze their structural characteristics and antioxidant activity,a microwave-assisted deep eutectic solvent(DES)extraction method was employed.The optimal extraction process was obtained through single-factor experiments and response surface methodology.The extracted polysaccharides were structurally characterized using Fourier transform infrared spectroscopy(FT-IR),X-ray diffraction(XRD),and field emission scanning electron microscopy(FE-SEM),and their antioxidant activity was analyzed.The results showed that the optimal extraction conditions for LJP were:DES with 20%water content(betaine to urea molar ratio of 1:2),solid-to-liquid ratio of 1:40(g/mL),microwave power of 426 W,extraction temperature of 56℃,and extraction time of 60 min.Under these conditions,the polysaccharide yield reached 5.96%,which was 52.04%higher than that obtained by hot water extraction.FT-IR analysis indicated that the structure of LJP extracted by microwave-assisted DES was diverse.XRD analysis revealed that LJP was an amorphous substance.FE-SEM showed that hot water extraction and microwave-assisted DES extraction had different effects on the structure of LJP.The LJP extracted by microwave-assisted betaine-urea exhibited good antioxidant activity,with ABTS+• and DPPH radical scavenging rates of 83.40%and 62.34%,respectively.This study provides a theoretical basis for developing and optimizing the high-value utilization of laminaria japonica polysaccharides.
郭丽;林宗伟;王喜庆;马雪;李杨;柴云雷;王鹏
绥化学院 食品与制药工程学院,黑龙江 绥化 152061绥化学院 食品与制药工程学院,黑龙江 绥化 152061绥化学院 食品与制药工程学院,黑龙江 绥化 152061绥化学院 食品与制药工程学院,黑龙江 绥化 152061绥化学院 食品与制药工程学院,黑龙江 绥化 152061绥化学院 食品与制药工程学院,黑龙江 绥化 152061绥化学院 食品与制药工程学院,黑龙江 绥化 152061
轻工纺织
海带多糖低共熔溶剂微波抗氧化活性
laminaria japonica polysaccharidesdeep eutectic solventmicrowaveantioxidant activity
《包装与食品机械》 2026 (1)
77-88,12
黑龙江省省属本科高等学校基本科研业务费基础研究项目(YWK10236210102)国家自然科学基金联合基金项目(U24A201282)
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