沙木蓼幼苗在不同盐度胁迫下的生理适应机制OA
Study on the physiological adaptation mechanism of Atraphaxis bracteata seedlings under different salinity stress
为探究沙木蓼(Atraphaxis bracteata)在盐胁迫条件下的生理响应与适应机制,本研究以沙木蓼幼苗为研究对象,设置了 5 个盐胁迫浓度梯度:0(CK)、50(T1)、100(T2)、150(T3)和 200 mmol·L-1(T4).测定了不同盐胁迫对沙木蓼幼苗气体交换参数、叶绿素荧光参数、抗氧化酶活性及渗透物质、非结构性碳水化合物等指标的影响;并通过相关性分析和主成分分析(PCA)揭示了沙木蓼幼苗在盐胁迫条件下的生理响应与适应机制.结果表明,随盐胁迫强度的增加,气孔导度、光合速率和蒸腾速率均呈下降趋势,光系统PSⅡ无显著变化趋势(P>0.05);丙二醛、脯氨酸、可溶性糖和淀粉含量整体呈上升趋势;抗氧化酶活性呈现先上升后下降趋势.由此可见,沙木蓼受到了不同程度抑制,但其可通过增加渗透调节物质含量和提高抗氧化酶活性的方式来应对盐胁迫造成的伤害.根据PCA排序可知,沙木蓼幼苗根部受到盐胁迫的危害较为严重.该结果可为沙木蓼在盐胁迫条件下的生理响应机制研究提供参考,为选育沙木蓼耐盐的造林品种提供理论依据.
To investigate the physiological response and adaptation mechanism of Atraphaxis bracteata under salt stress conditions,this study focused on A.bracteata seedlings and established five salt stress concentration gradients:0(CK),50(T1),100(T2),150(T3),and 200 mmol·L-1(T4).The effects of different salt stresses on gas exchange parameters,chlorophyll fluorescence parameters,antioxidant enzyme activity,osmotic substances,non-structural carbohydrates,and other indicators of A.bracteata seedlings were determined.Through correlation and principal component analyses,the physiological responses and adaptation mechanisms of A.bracteata seedlings under salt stress conditions were revealed.The results showed that with an increase in salt stress intensity,stomatal conductance,photosynthetic rate,and transpiration rate all showed a decreasing trend,whereas photosystem PSⅡ showed no significant change.The malondialdehyde,proline,soluble sugar,and starch contents all showed an overall upward trend,with antioxidant enzyme activity showing a trend of initial increase followed by a decrease.From these findings,it is considered that A.bracteata growth was inhibited to varying degrees,but seedlings can cope with the damage caused by salt stress by increasing the content of osmoregulatory substances and enhancing antioxidant enzyme activity.According to the PCA ranking,it can be concluded that the roots of A.bracteata seedlings are more severely affected by salt stress.These results provide a basis for studying the physiological response mechanism of A.bracteata under salt stress conditions and provide a theoretical foundation for breeding salt-tolerant afforestation varieties of A.bracteata.
刘源;何彩;晋敏;胡芳;刘伟;金娜;李毅
甘肃农业大学林学院,甘肃 兰州 730070甘肃农业大学林学院,甘肃 兰州 730070||武威市林业科学研究院,甘肃 武威 733000武威市林业科学研究院,甘肃 武威 733000武威市林业科学研究院,甘肃 武威 733000武威市林业科学研究院,甘肃 武威 733000武威市林业科学研究院,甘肃 武威 733000甘肃农业大学林学院,甘肃 兰州 730070
沙木蓼盐胁迫光合特性渗透调节物质抗氧化酶活性相关性分析主成分分析
Atraphaxis bracteatasalt stressphotosynthetic characteristicsosmotic regulation substancesantioxidant enzyme activitycorrelation analysisprincipal component analysis
《草业科学》 2026 (1)
65-75,11
甘肃省科技计划资助项目(24JRRH007、21JR7RH893)武威市市级科技计划项目(WW24B01NY045)甘肃省青年人才项目(2023LQGR46)甘肃省第二批陇原青年英才项目(甘肃省委人才领导小组[2023]11号)
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