两个金银花品种叶片中转录因子MYB家族成员的差异分析OA

Taking leaves of the honeysuckle new varieties'Zhengyinhua No.3'and'Fenghua No.1'as the test materials,bioinformatics methods were employed to analyze the physicochemical properties,secondary structure,motif comparison,phylogenetic relationships,and functional annotations of MYB transcription factors in honeysuckle.The study examined the expression differences of MYB transcription factors in the leaves of these two honeysuckle varieties,in order to provide reference for exploration of the differences and functional characterization of MYB family members in the leaves of these two honeysuckle varieties.The results showed that there were 52 members of the MYB transcript factor family in the leaves of two varieties,mainly distributed on chromosomes 5 and 9.All proteins showed hydrophilicity,with 23 proteins being acidic and 29 proteins being alkaline.The motif analysis showed that 49 transcription factors included Motif 3,which had the highest frequency of occurrence,while all 34 transcription factors contained Motif 3,Motif 2,and Motif 1.The heat map indicated that there were 14 genes with significant differences in expression levels between the two varieties.Combined with GO functional annotation,it was found that these 14 differentially expressed genes might be related to the growth and development in Lonicera japonica.