活络效灵丹对骨骼肌钝挫伤大鼠炎症指标及组织形态学的影响OA

Objective:To observe the effects of Huoluo Xiaoling Dan(HLXLD)on leg circumference,gait,histomorphology and inflammatory indicators in rats with skeletal muscle contusion(SMC),and to investigate the efficacy of different concentrations of HLXLD on SMC.Methods:Forty healthy male Sprague-Dawley(SD)rats were routinely fed for 1 week,and randomly divided into 5 groups(n=8 each):blank group,model group,low-concentration HLXLD group,high-concentration HLXLD group,and diclofenac sodium group.SMC model was established in all rats except the blank group.Gait data were collected using the CatWalk gait analysis system before modeling.The affected leg circumference was measured at 12 h after modeling.Drug intervention was initiated 24 h after modeling:rats in low-and high-concentration HLXLD groups were intragastrically administered HLXLD suspension at 0.083 g/mL and 0.332 g/mL,respectively;the diclofenac sodium group was given 9 mg/mL diclofenac sodium solution;blank and model groups received equal volume of normal saline.All interventions were given twice daily for 1 week at 10 mL/kg·d.After intervention,the affected leg circumference was measured,gait data were collected,gastrocnemius muscle morphology was observed by hematoxylin-eosin(HE)staining,and the levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the blank group,the model group showed significantly increased leg circumference difference(P<0.01),significantly decreased average swing speed and average standing phase(P<0.01),disorganized muscle fibers,enlarged intercellular space,massive erythrocyte and inflammatory cell infiltration,as well as significantly elevated TNF-α and IL-6 levels(P<0.01).Compared with the model group,low-concentration HLXLD,high-concentration HLXLD and diclofenac sodium groups exhibited significantly reduced leg circumference difference(P<0.01),increased average swing speed and standing phase(P<0.01),alleviated muscle fiber disarrangement and inflammatory cell infiltration,and markedly decreased TNF-α and IL-6 levels(P<0.01).Compared with the low-concentration HLXLD group,the high-concentration HLXLD group showed further decreased leg circumference difference(P<0.01),increased swing speed and standing phase(P<0.01),tighter muscle fiber arrangement,smaller intercellular space,less inflammatory cell and erythrocyte aggregation,and lower TNF-α and IL-6 levels(P<0.01).Conclusion:HLXLD can significantly alleviate inflammation,repair skeletal muscle structure,and improve motor function in SMC rats.The therapeutic effect of HLXLD on SMC may be positively correlated with its concentration.