牛膝多糖对激素性股骨头坏死大鼠骨代谢的影响OA

Objective:To investigate the effects of Achyranthes bidentata polysaccharide on bone metabolism in steroid-in-duced osteonecrosis of the femoral head(SONFH)rats and to explore whether its mechanism is associated with the bone morphogenetic protein-2(BMP-2)/Smad5/Runt-related transcription factor 2(Runx2)signaling pathway.Methods:The successfully constructed SONFH rats were randomly divided into the following groups:model group,low-dose Achyran-thes bidentata polysaccharide group(2.0 g/kg),medium-dose Achyranthes bidentata polysaccharide group(4.0 g/kg),high-dose Achyranthes bidentata polysaccharide group(8.0 g/kg),and high-dose Achyranthes bidentata polysaccharide+BMP-2 inhibitor Noggin group(8.0 g/kg Achyranthes bidentata polysaccharide+100 ng/kg Noggin),with 12 rats in each group.Another 12 rats were considered as the control group.An ELISA kit was used to detect bone metabolism indicators in rats.Dual-energy X-ray absorptiometry was performed to measure bone mineral density(BMD)in rats.HE staining was performed to examine the pathological morphology of rat femoral head tissue.Western Blot was performed to detect proteins related to the BMP-2/Smad5/Runx2 signa-ling pathway in rat femoral head tissue.Results:In the con-trol group,the morphology and structure of the rat femoral head were normal.The spatial structure of the bone trabeculae in the model group was disrupted,with thin and sparse bone trabeculae and increased gaps.The model group had lower levels of ALP,PINP,BGP,BMD,BMP-2,p-Smad5/Smad5,and Runx2 than control group,and higher TRAP,NTx,and empty bone resorption rate than the control group(P<0.05).The low-dose,medium-dose,and high-dose Achyranthes bidentata polysaccharide groups exhibited higher levels of ALP,PINP,BGP,BMD,BMP-2,p-Smad5/Smad5,and Runx2,and lower levels of TRAP,NTx,and bone resorption pit rate than the model group,with the morphology of bone trabecu-lae showing varying degrees of improvement(P<0.05).The high-dose Achyranthes bidentata polysaccharide+Noggin group had lower ALP,PINP,BGP,BMD,BMP-2,p-Smad5/Smad5,and Runx2,and higher TRAP,NTx,and empty bone resorption rate than the high-dose Achyranthes bidentata polysaccharide group,and the degree of morphological damage to the bone trabeculae was more severe(P<0.05).Conclusion:Achyranthes bidentata polysaccharide can improve bone metabolism in SONFH rats,and the mechanism may be related to the activation of BMP-2/Smad5/Runx2 pathway.