参骨颗粒治疗绝经后骨质疏松的作用机制OA

Objective:The effect of Shengu granules on intestinal microflora and inflammatory factors in ovariectomized rats was studied based on the gut-bone axis to reveal its mechanism in treating postmenopausal osteoporosis.Methods:Thirty-six female rats were randomly divided into three groups:Sham group,model group,and Shengu granules-treated group.After 12 weeks of oral gavage with Shengu granule solution,bone mineral density(BMD)and bone micros-tructures were measured by Micro-CT,and bone turnover markers(PINP,β-CTX)and inflammatory cytokines(IL-10,IL-17,TGF-β,TNF-α)were detected by ELISA.The protein ex-pression levels of osteoprotegerin(OPG)and receptor acti-vator of NF-κB ligand(RANKL)were detected by Western Blot,and the intestinal microbiota was analyzed by 16S rRNA amplification and sequencing.Results:Compared with the Sham group,the Shengu granules-treated group exhibited decreased BMD and BV/TV levels and increased Tb.Sp;combined with bone microstructure analysis,these findings indi-cate that ovariectomized rats developed osteoporotic-like bone changes.Compared with the model group,the Shengu gran-ules-treated group showed significantly higher protein expression levels of PINP,IL-10,TGF-β,and OPG,as well as signifi-cantly lower expression levels of β-CTX,IL-17,TNF-α,and RANKL.All these differences were statistically significant(P<0.05),indicating that Shengu granules treatment reduces bone loss and improves bone microstructure in the Shengu granules-trea-ted group.Furthermore,compared with the model group,the Shengu granules-treated group demonstrated increased gut microbio-ta community diversity and improved community composition(P<0.05),suggesting that Shengu granules can alleviate gut microbiota dysbiosis associated with osteoporosis.Conclusion:Shengu granules can improve postmenopausal osteoporosis,and the underlying mechanism may be related to the RANKL/OPG pathway mediated by gut microbiota and inflammatory factors.