基于转录组数据的3种水蛭密码子使用偏好性分析OA

Objective:To analyze the usage patterns and influencing factors of codons of transcriptome expression genes in different Hirudo,and to explore the possible correlations between gene sequences and functional proteins,so as to provide the indispensable key data and theoretical support for the practical gene engineering of large-scade production of Hirudo active proteins.Methods:Perl lan-guage and CondonW1.4.2 software were used to systematically analyze the codon usage preferences and causes of the transcriptome coding sequences of Hirudo nipponica,Hirudinaria manillensis and Whitmania pigra.Results:The three types of Hirudo transcriptome codons had certain similarities in usage patterns.In terms of total base composition,the A/T content was relatively high and the G/C content was relatively low.However,the preferred codons at the end were not consistent.There were two common optimal codons among the three(AGA encoding Arg and GCA encoding Ala).The optimal codons of Hirudo nipponica preferred to end with G/C,while those of Whitmania pigra and Poecilobdella manillensis preferred to end with A/T.Neutral plot analysis indicated that the matural selection played a decisive role in shaping the preference for the use of codons in the three Hirudo species.Conclusion:This study clearly explains the codon usage characteristics and evolutionary dynamics at the genetic level of three types of medicinal Hirudo.This result provides basic for the subsequent biosynthesis research on the hirudin and its ralated drugs.