苍柏丹防治骨科术后下肢深静脉血栓形成及炎症反应的实验研究OA

Objective:To evaluate the effects of Cangbai Dan on deep vein thrombosis(DVT)formation and inflammation after major orthopedic surgery.Methods:Fifty rats were ran-domly assigned to five groups(n=10 each):blank,model,low-dose Cangbai Dan,high-dose Cangbai Dan,and positive drug group.Except for the blank group,all rats underwent DVT modeling.From day 5 post-surgery,rats received intra-gastric administration of Cangbai Dan(6.84 g/kg or 13.68 g/kg,twice daily),saline(2.5 mL,twice daily),or saline com-bined with low-molecular-weight heparin calcium(0.3 mL,subcutaneously,once daily)for five consecutive days.His-topathological changes in the great saphenous vein were evaluated by hematoxylin-eosin(HE)staining.Expression of IκBα and p65 proteins was assessed by immunohistochemistry and Western Blot.Serum IL-6,IL-8,and HMGB1 levels were measured by ELISA.Results:HE staining showed that Cangbai Dan and the positive drug protected the vascular endotheli-um,with superior effects in the high-dose group.ELISA demonstrated that IL-6,IL-8,and HMGB1 levels were lowest in the blank group and highest in the model group,with dose-dependent reductions in the Cangbai Dan groups.No significant difference was observed between the high-dose Cangbai Dan group and the positive drug group(P>0.05).Western Blot and immunohistochemistry showed consistent trends in IκBα and p65 expression.Conclusion:Cangbai Dan prevents post-operative lower limb DVT by inhibiting NF-κB pathway activation,downregulating NF-κB p65 and IκBα expression,and attenuating inflammatory responses.These findings support its therapeutic potential in the prevention of thrombosis after orthopedic surgery.