ASAP-MS结合化学计量学鉴别胆类药材及掺伪品OA

Objective:To establish a rapid identification model for bile medicinal materials from different sources and the identifi-cation of Suis Fellis Pulvis in bear bile powder based on atmospheric pressure solid-phase analysis probe tandem single quadrupole mass spectrometry(ASAP-MS)combined with stoichiometric analysis,in order to seek new plans to solve the problem of authenticity identification of bile medicinal materials.Methods:The mass spectrometry information of bear bile powder,cow bile powder,snake bile powder,Suis Fellis Pulvis,sheep bile powder,rabbit bile powder,chicken bile powder,duck bile powder and goose bile powder was col-lected by ASAP-MS.Combined with stoometrics,principal component-linear discriminant analysis(PCA-LDA)model was constructed to separately evaluate the reliability of the detection results of different bile types samples.Results:The PCA-LDA model could effectively distinguish 10 kinds of bile medicinal materials.The internal validation recognition rate of the model was 98.35%,and the external vali-dation recognition rate was 97.70%.In addition,this method could also identify the adulteration of Suis Fellis Pulvis in bear bile pow-der,with a minimum detection limit of 3%.The internal verification recognition rate of the model was 99.51%.The recognition rate of external verification was 99.22%.Conclusion:The ASAP-MS combined with PCA-LDA model established in this study can efficiently and accurately achieve rapid identification of adulteration of different bile medicinal materials and Suis Fellis Pulvis,providing a feasible new method for the authenticity identification of bile medicinal materials.