皂角刺乙酸乙酯部位化学成分及抗炎活性研究OA北大核心CSTPCD

Objective:To isolate the chemical components from the ethyl acetate part of Gleditsia sinensis calthrop and explore the traditional medicinal material basis of Gleditsia sinensis calthrop.Methods:The ethyl acetate fraction of Gleditsia sinensis calthrop was systematically isolated by various column chromatography techniques and semi-preparative high performance liquid chromatography,and the structures were identified by spectroscopic methods.Molecular docking of the isolated compounds with inflammation,vascular perme-ability,key tumor target proteins Akt,BRD4,ST2,PKC,TKT was performed using Discovery Studio software.The anti-inflammatory ac-tivity was verified by the Griess method to identify the potential active ingredients for the treatment of"carbuncle and deep-rooted car-buncle"in their traditional efficacy.Results:14 compounds were isolated from the ethyl acetate part of Gleditsia sinensis calthrop and identified as lariciresinol(1),balanophonin(2),dihydrodehydrodiconifenyl alcohol(3),rasidasinⅠ(4),3-[2-(4-hydroxyphenyl)-3-hydroxymethyl-2,3-dihydro-1-benzofuran-5-yl]propan-1-ol(5),polystachyol(6),erythro-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(7),3,3′-dimethoxy-4,8′-oxyneoligna-9,4′,7′,9′-tetraol(8),idaeusinol A(9),salicifoliol(10),evofolin B(11),threo-2,3-bis-(4-hydroxy-3-methoxypheyl)-3-methoxypropanol(12),p-hydroxycinnamaldehyde(13),3,3-di-(4-hydroxy-3-methoxyphenyl)-propylene-1,2-diol(14),respectively.Conclusion:All compounds are isolated from Gleditsia genus for the first time.The results of molecular docking show that lignans have good affinity with inflammatory abscess related proteins.The results of anti-inflammatory activity validation show that compound 1 has some anti-inflammatory activity.