基于网络药理学探讨小柴胡汤合瓜蒌薤白干预胃食管反流病伴胸闷的作用机制OA

Objective To explore the potential mechanism of Xiaochaihu decoction combined with Gualou and Xiebai in intervening of gastroesophageal reflux disease(GERD)with chest tightness based on network pharmacology.Methods Using TCMSP,HERB,and BATMAN-TCM data base as data sources,the active components of Xiaochaihu decoction combined with Gualou and Xiebai were screened,and the related target points were further predicted.The target information of all components was standardized to obtain a set of potential target points.Simultaneously,targets related to GERD and chest tightness were collected,and the intersection operation between drug targets and disease targets were performed to obtain the intersection targets.A multidimensional network of drugs-component-targets was constructed,and core active components were screened.Protein.Protein interaction networks were constructed,and key targets were screened.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were conducted to screen significant biological processes and signaling pathways.A drug-disease-target-pathway network was constructed,and core targets were identified.Finally,molecular docking validation was performed.Results The compound's primary bioactive components include quercetin,kaempferol,and baicalin.Its key targets are enriched in mitogen-activated protein kinase 3,serine/threonine kinase 1,and nuclear factor κB subunit 1.The signaling pathways focus on lipid-related atherosclerosis,the phosphatidylinositol 3-kinase/protein kinase B signaling pathway,and the advanced glycation end product receptor signaling pathway in diabetic complications.Docking results indicated that the core components exhibit low binding affinity and conformational stability with target proteins.Conclusion This compound may alleviate GERD with chest tightness by modulating inflammatory-immune imbalance,regulating glucose-lipid metabolism disorders,and reducing visceral hypersensitivity.