基于"因虚致病"理论和孟德尔随机化探讨虚弱与肝酶相关性OA

Objective To investigate the causal relationship between frailty and liver enzyme levels based on the theory of"illness caused by deficiency"in TCM and Mendelian randomization.Methods Using data from genome-wide association studies(GWAS)databases,specific single nucleotide polymorphisms(SNPs)related to frailty and liver enzyme levels were identified and used as instrumental variables.Mendelian randomization analysis was conducted using three methods:inverse variance weighted(IVW),MR-Egger regression,and weighted median,to evaluate the correlation between frailty and liver enzyme levels.Sensitivity analysis and pleiotropy tests were performed,including Cochran's Q test,MR-Egger test,MR-PRESSO test,and leave-one-out analysis,in order to ensure the reliability and robustness of the results.Results After analyzing data from the UK Biobank(UKB)database,a significant positive causal relationship was found between frailty and ALT(Beta=0.095,95%CI:0.036-0.153,P=0.001).The significant correlation was identified between frailty and ALP(Beta=0.144,95%CI:0.054-0.235,P=0.002),frailty and GGT(Beta=0.157,95%CI:0.072-0.242,P<0.001),and frailty and AST(Beta=0.092,95%CI:0.023-0.160,P=0.008).Furthermore,results from the GWAS Catalog database also supported the presence of a positive causal relationship between frailty and ALT,ALP,and GGT(P<0.05).Conclusion There is a significant correlation between frailty and liver enzymes,enriching the biological connotation of the theory of"illness caused by deficiency".At the same time,it is suggested that frailty should be considered as a potential risk factor for elevated liver enzyme levels in clinical practice.