滋阴润燥丸对干燥综合征模型小鼠颌下腺炎症及功能作用研究OA

AIM:To investigate the effect of ZiYin RunZao(ZYRZ)pill on the inflammation and func-tion of submandibular gland in mice modeled with Sjögren's syndrome.METHODS:Forty 8-week-old NOD/Ltj mice were randomly divided into five groups:model group,western medicine group,and the high,middle and low dosage groups of ZYRZ,with eight mice in each group.Another 8 ICR mice were blank group.The intervention was started af-ter 1 week of acclimatization feeding.In the high,medium and low dose groups,1.78,0.89 and 0.45 mg/kg of ZYRZ pills were administered by gavage,respectively;in the western medicine group,60 mg/kg of hydroxychloroquine was administered;and in the blank group and the model group,equal amounts of sterile distilled water were adminis-tered by gavage once a day for eight consecutive weeks.The body weight and water intake of the mice were monitored at weeks 0,2,4,6 and 8.At the end of the intervention,mice submandibular gland and serum were taken,and the histopatholo-gy of submandibular gland was observed by HE staining;the levels of inflammatory cytokines IFN-γ,IL-10,and TNF-α were detected in serum by ELISA;the levels of AQP5 and M3R mRNA were detected in submandibular gland by RT-qPCR;the levels of AQP5 and M3R mRNA in submandibular gland were detected in submandibular gland by Western blot;and the levels of AQP5 and M3R mRNA in sub-mandibular gland were detected in submandibular gland by RT-qPCR.AQP5,M3R protein expression;immunohistochemistry to detect the level of AQP5 protein expression in the submandibular gland tis-sue of mice.RESULTS:Compared with the normal group,mice in the model group showed no signifi-cant changes in body weight,significantly increased water intake(P<0.05),significantly higher expres-sion of IFN-γ and TNF-α in serum of mice(P<0.05),and significantly lower expression of IL-10(P<0.05);significantly lower expression of mRNA and protein of AQP5 and M3R(P<0.05);compared with the model group,the western blot and the ZYRZ Pill in high school and the western blot were detected in the submaxillary gland tissues of mice;immunohis-tochemical method was used to detect the expres-sion level of AQP5 in the submaxillary gland tissues.Compared with the model group,mice in the west-ern medicine group and the high school dose group of ZYRZ consumed significantly less water(P<0.05),the expression of IFN-γ and TNF-α in serum was sig-nificantly reduced(P<0.05),the expression of IL-10 was significantly elevated(P<0.05),and the expres-sion of AQP5,M3R mRNA,and protein was signifi-cantly elevated(P<0.05).CONCLUSION:ZYRZ pill can reduce the inflammatory reaction of sub-mandibular gland in mice with Sjögren's syndrome model and improve the secretion function of sub-mandibular gland.