沼液对番茄生长和土壤细菌群落及功能的影响OA

To systematically investigate the effects of biogas slurry application on soil-plant interactions,tomato variety'Provence'was taken as the research object.A pot experiment were conducted with six treatments:T1 treatment(V(water)∶V(biogas slurry)=1∶1),T2 treatment(V(water)∶V(biogas slurry)=2∶3),T3 treatment(V(water)∶V(biogas slurry)=1∶2),T4 treatment,(V(water)∶V(biogas slurry)=2∶5),T5 treatment(full application of biogas slurry),and CK(no biogas slurry,distilled water only)(CK).The effects of different concentrations of biogas on tomato growth,nutrient uptake,soil properties and soil microbial community were determined.The results showed that:1)Under the biogas slurry application treatments(T1-T5),the aboveground dry matter weight of tomato ranged from 3.97 to 5.05 g,while the dry matter content of CK was 3.97 g.The aboveground dry matter under different biogas slurry treatments was higher than that of CK.The contents of total nitrogen and total phosphorus in shoots under different treatments were significantly higher than CK(P＜0.05),and the contents of total nitrogen and total phosphorus in shoots under T5 biogas slurry treatment were the highest,which were 32.66 and 4.26 g/kg,representing increases of 67.90％and 51.57％,respectivley.2)Under T3 and T5 treatments,the soil conductivity increased with the increase of biogas slurry concentration,and the variation ranged from 156 to 746 μS/cm.After biogas slurry application,the soil pH decreased significantly(P＜0.05).The contents of nitrate nitrogen,available phosphorus and available potassium in soil increased significantly with the increase of biogas slurry concentration,and the increase of nitrate nitrogen content was the most significant(P＜0.05).The nitrate nitrogen content was the highest under T5 treatment,reaching 337.4 mg/kg(P＜0.05).The contents of sodium ion,potassium ion,chloride ion and sulfate ion in soil increased significantly with the increase of biogas slurry concentration(P＜0.05),and the content was the highest at the original concentration.3)The application of original biogas slurry significantly increased the activities of leucine aminopeptidase(LAP),β-N-Acetylglucosaminidase(NAG)and acid phosphatase(AP)in the soil(P＜0.05).4)PCoA analysis revealed that the soil bacterial diversity using the original biogas slurry was significantly higher than that of the control,and the bacterial community composition differed significantly compared to other treatments(P＜0.05).In summary,adding biogas slurry to soil can increase tomato biomass and soil nutrient content.Among them,applying raw biogas slurry can enhance soil enzyme activities related to nitrogen and phosphorus cycling,increase soil bacterial abundance,and improve bacterial community composition.This study provided an important theoretical basis for the scientific application of biogas slurry in tomato growth.