蒙古冰草MADS-box基因家族鉴定及其对干旱胁迫的响应OA
Identification of MADS-box gene family in Agropyron mongolicum Keng and its response to drought stress
为挖掘蒙古冰草(Agropyron mongolicum Keng)中与干旱胁迫响应相关的AmMADSs,以25％(0.0417 mol/L)PEG 6 000模拟干旱胁迫处理得到的蒙古冰草转录组测序数据Unigenes为对象,筛选出12个MADS-box家族基因,利用生物信息学方法对其理化性质、亚细胞定位、保守基序、系统进化树及蛋白质二、三级结构进行分析,并结合qRT-PCR技术检测其在25％(0.041 7 mol/L)PEG 6 000处理0(对照,CK)、1.0、3.0、5.0、7.0 d和复水1.0d后的MADS-box家族基因的表达模式.结果表明:筛选出的12个蒙古冰草MADS-box转录因子,蛋白质分子量在7 263.38～31 349.53 u,编码的氨基酸长度为65～273个,蛋白质理论等电点(pI)在5.43～11.16,其中3个MADS-box转录因子属于酸性蛋白、9个MADS-box转录因子属于碱性蛋白;12个蒙古冰草MADS-box基因编码蛋白质均定位于细胞核,不含有跨膜结构域,存在大量的磷酸化位点,蛋白质二、三级结构以无规则卷曲和α-螺旋为主.系统进化树分析发现,蒙古冰草AmMADS26-1、AmMADS26-2、AmMADS29-2、AmMADS47和AmMADS55基因与水稻已知具有抗旱功能的MADS-box家族蛋白的同源关系较近.qRT-PCR结果表明,在模拟干旱处理(25％PEG 6 000)后,A mMADS26-1、A mMADS26-2、A mMADS29-2 和 AmMADS47在干旱处理1.0d后的表达水平较0d(CK)增加1.55～4.55倍,其中AmMADS26-1和AmMADS47的表达模式在干旱处理第1-7天虽呈下调趋势,但其表达水平均高于CK的表达水平;而AmMADS55在干旱处理3.0和5.0 d后的表达水平分别比CK高1.57和2.28倍.综上,基于对蒙古冰草MADS-box家族基因的生物信息学分析及表达模式鉴定,筛选得到5个响应干旱胁迫的关键基因,分别是AmMADS26-1、AmMADS26-2、AmMADS29-2、AmMADS47 和 AmMADS55.
To identify drought-related AmMADSs in Agropyron mongolicum Keng,the unigenes obtained from transcriptome sequencing of A.mongolicum under 25％(0.041 7 mol/L)PEG 6 000-simulated drought stress was used as the research object.A total of twelve MADS-box family genes were screened.Bioinformatics methods were used to analyze their physicochemical properties,subcellular localization,conserved motifs,phylogenetic tree,and protein secondary and tertiary structures.Additionally,qRT-PCR technology was employed to examine the expression patterns of MADS-box family genes at 0(CK),1.0,3.0,5.0,7.0 d after 25％(0.0417 mol/L)PEG 6 000 treatment and 1.0 d after rehydration.The results show that:The protein molecular weights of the twelve selected MADS-box transcription factors from A.mongolicum ranges from 7 263.38 to 31 349.53 u,and the length of encoded amino acids ranges from 65 to 273.The theoretical isoelectric points(pl)of the proteins ranges from 5.43 to 11.16.Among which,three MADS-box transcription factors are acidic proteins and nine are basic proteins.The proteins encoded by all twelve A.mongolicum MADS-box genes are localized in the nucleus.They lack transmembrane domains,and contain numerous phosphorylation sites,and their secondary and tertiary structures are primarily composed of random coils and a-helices.Phylogenetic analysis revealed that the A.mongolicum genes,AmMADS26-1,AmMADS26-2,AmMADS29-2,AmMADS47,and AmMADS55 are closely related to known drought-resistant MADS-box family proteins in rice.qRT-PCR results showed that after simulated drought treatment(25％PEG 6,000),the expression levels of AmMADS26-1,AmMADS26-2,AmMADS29-2,and AmMADS47 increased by 1.55 to 4.55 times at 1.0 d of drought treatment compared to the CK.Among them,the expression patterns of AmMADS26-1 and AmMADS47 showed a downward trend from day 1 to 7 under drought treatment,but their expression levels were still higher than those of CK.In contrast,the expression level of AmMADS55 was 1.57 and 2.28 times higher than that of CK after 3.0 and 5.0 d of drought treatment respectively.In summary,based on bioinformatics analysis and expression pattern identification of the MADS-box gene family in A.mongolicum,this study identified five key genes responsive to drought stress,namely AmMADS26-1,AmMADS26-2,AmMADS29-2,AmMADS47,and AmMADS55.
杜晓红;聂虎帅;马宇;房永雨;吕二锁;翟永青;梅新艳;赵彦;马艳红
内蒙古农业大学农学院,呼和浩特 010019内蒙古农业大学农学院,呼和浩特 010019内蒙古农业大学农学院,呼和浩特 010019||内蒙古自治区农牧业科学院作物科学研究所,呼和浩特 010031内蒙古自治区农牧业科学院生物技术研究所,呼和浩特 010031内蒙古自治区农牧业科学院作物科学研究所,呼和浩特 010031内蒙古农业大学农学院,呼和浩特 010019内蒙古农业大学农学院,呼和浩特 010019内蒙古农业大学草业学院,呼和浩特 010018内蒙古农业大学农学院,呼和浩特 010019
农业科技
蒙古冰草MADS-box生物信息学分析干旱胁迫
Agropyron mongolicum KengMADS-boxbioinformatics analysisdrought stress
《中国农业大学学报》 2026 (4)
29-43,15
国家自然科学基金(32360338)
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