紫锥菊提取物对八眉三元猪屠宰性能、肌肉抗氧化、肉品质和脂质代谢的影响OA

The aim of this experiment was to investigate the effects of Echinacea extract on slaughter performance,muscle antioxidant capacity,meat quality in eight-brow ternary pigs.In the present experiment,48 Bamei ternary pigs were randomly divided into 4 groups with 3 replicates each and 4 pigs in each replicate.The experimental period was 42 days,including 7 days of pre-feeding period and 35 days of main test period.The control group was fed a basal diet,while the experimental groups T1,T2 and T3 were supplemented with Echinacea purpurea extract at 5,10 g/kg and 15 g/kg,respectively,on top of the control diet.The results showed that:(1)Compared with CON group,slaughter rate of each experimental group were significantly increased(P<0.05).(2)Compared with the CON group,the malondialdehyde(MDA)content was significantly reduced in the test group,total antioxidant capacity(T-AOC)content was significantly increased in the T1 group,T-AOC,superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)content was significantly increased in the T2 group,and CAT content was significantly increased in the T3 group(P<0.05).(3)The shear force of the longest dorsal muscle was significantly lower in the T2 group compared with the CON group(P<0.05).(4)The content of lysine,threonine,glycine,aspartic acid,hydroxyproline,and cysteine was significantly higher in the experimental group compared with the CON group(P<0.05).(5)Compared with the CON group,the saturated fatty acid(SFA)content was significantly lower and the monounsaturated fatty acid(MUFA)content was significantly higher in the T2 group,and the MUFA content was significantly higher in the T3 group(P<0.05).(6)Liver fat:compared with the CON group,the protein levels of ACC and PPARγ were significantly reduced in the T1 group,the mRNA and protein levels of CPT1 were significantly increased,and the mRNA level of HSL was significantly increased in the T2 group(P<0.05);The mRNA and protein levels of PPARγ were significantly reduced,the mRNA level of SREBP1 was significantly reduced,and the mRNA and protein levels of ACC levels were significantly reduced,and the mRNA and protein levels of HSL and CPT1 were significantly increased(P<0.05);In the T3 group,the mRNA and protein levels of PPARγ were significantly reduced,the protein level of ACC was significantly reduced,the mRNA level of SREBP1 was significantly reduced,and the mRNA and protein levels of CPT1 were significantly increased(P<0.05).Subcutaneous fat:compared with the CON group,the mRNA levels of SREBP1 and PPARγ were significantly reduced in the T1 group,the mRNA and protein levels of CPT1 were significantly increased,and the protein level of HSL was significantly increased(P<0.05);In the T2 group,the mRNA and protein levels of ACC,SREBP1,and PPARγ were significantly reduced,the mRNA and protein levels of HSL were significantly increased,and the mRNA level and protein level of CPT1 were significantly increased(P<0.05);In the T3 group,the mRNA level of SREBP1 was significantly reduced,the mRNA and protein levels of PPARγ and ACC were significantly reduced,and the mRNA and protein levels of HSL and CPT1 were significantly increased(P<0.05).In conclusion,Echinacea extract improved meat quality by increasing growth performance,slaughter performance,antioxidant capacity of the longest back muscle,muscle quality,amino acid and fatty acid content,and regulated the expression of lipid metabolism factors to prevent lipid metabolism disorders caused by fat deposition in eight-brow ternary pigs.Under the conditions of this experiment,the best effect was achieved when the concentration of Echinacea extract-chicoric acid was 10 g/kg.