新型呕吐毒素降解菌JM-3的全基因组测序和生物信息学分析OA

In this experiment a strain of bacteria JM-3 from soil samples in wheat fields and with high efficiency in degrading deoxynivalenol(DON)were used.The third-generation sequencing technology was used to measure the whole genome sequence of the JM-3 strain.The genome was assembled,analyzed,and circle mapped using software such as HGAP,Canu,CGView.In addition,coding gene prediction,functional annotation,systematic evolution analysis,and collinearity analysis were performed using software of Glimmer,KOBAS,MEGA,and Mauve.The whole genome analysis results showed that the JM-3 genome was approximately 4.7 Mb in length,with a GC content of 63.9％.4534 coding genes were predicted.By comparing GO,COG,KEGG,and CAZy databases,2217,3304,2256,and 478 genes were annotated,respectively.Systematic evolutionary analysis showed that the JM-3 strain had the closest evolutionary relationship with Devosia bacteria.Based on our previous morphological,physiological,and biochemical analysis results,it was identified as Devosia sp.The results of comparative genome analysis indicated that,although JM-3 strain had significant DON degradation ability as well as previously reported Devosia sp.A16 and Devosia sp.D6-9,its genome existed multiple instances of gene segment insertion/deletion,rearrangement,and translocation,indicating that JM-3 was a new DON degrading strain.These results provide important data for further exploration of DON degrading enzymes from the JM-3 genome.