妊娠合并乙肝病毒感染患者血清DNMT1、IP-10和IFN-γ表达与母婴不良结局的相关性分析OA

Objective:To investigate the relationship between serum DNA methyltransferase 1(DNMT1),interferon gamma-inducible protein-10(IP-10),and IFN-γ expressions and adverse maternal-infant outcomes in pregnant women with hepatitis B virus(HBV)infection.Methods:A total of 107 pregnant women with HBV infection admitted to The First People's Hospital of Shangqiu from January 2022 to January 2025 were enrolled as the infected group,and 72 healthy pregnant women undergoing prenatal examina-tion and delivery during the same period were selected as the control group.Serum levels of DNMT1,IP-10,and IFN-γ,as well as the incidence of adverse maternal-infant outcomes,were compared between the two groups.According to pregnancy outcomes,the infect-ed group was further divided into an adverse maternal-infant outcome group(n=59)and a favorable maternal-infant outcome group(n=48).Univariate and multivariate Logistic regression models were used to identify independent risk factors for adverse maternal-infant outcomes.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the predictive efficiency of serum DN-MT1,IP-10,and IFN-γ for adverse maternal-infant outcomes in pregnant women with HBV infection.Results:DNMT1 and IP-10 lev-els were significantly higher in the infected group than those in the control group(P<0.05),while IFN-γ level was significantly lower than in the control group(P<0.05).The incidence rates of intrahepatic cholestasis of pregnancy,gestational diabetes mellitus,neona-tal asphyxia,and preterm birth were also significantly higher in the infected group(P<0.05).Compared with the favorable outcome group,the adverse outcome group exhibited significantly higher positive rate of HBV viral load,levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),DNMT1,and IP-10,as well as significantly lower level of IFN-γ(P<0.05).Multivariate analysis showed that HBV viral load≥1.0×10³ U/mL,elevated serum AST,ALT,TBIL,DNMT1,and IP-10 were independent risk factors for adverse maternal-infant outcomes,while IFN-γ was a protective factor(P<0.05).ROC curve analysis demonstrated that DNMT1,IP-10,IFN-γ alone and in combination all showed statistically significant predictive value for adverse maternal-infant outcomes(P<0.05).The combined detection achieved an AUC of 0.960(95%CI:0.929～0.990),with a sensitivity of 0.831 and a specificity of 0.958,indicating high predictive performance.Conclusion:Increased serum levels of DNMT1 and IP-10 and decreased level of IFN-γ are significantly associated with adverse maternal-infant outcomes in pregnant women with HBV infec-tion.DNMT1 and IP-10 are independent risk factors for adverse maternal-infant outcomes,whereas IFN-γ serves as a protective factor.The combined detection of the three indicators exhibits better predictive efficiency than any single indicator.