鸡舍养殖模式与优质肉鸡肠道形态及微生物群落的关联OA

To clarify the intestinal morphology and microbiome changes of high-quality broilers in different types of chicken houses,240 healthy 1-day-old high-quality broilers of the L line were selected and randomly divided into two groups,which were raised in a closed environmental control cabin(Group Ⅰ)and a common semi-closed chicken coop(Group Ⅱ).At the 15th week of age,24 roosters in each group were selected and slaughtered for intestinal morphology determination,and the microbial diversity in cecum contents was analyzed by 16S rDNA sequencing.The results showed as follows:1)At 15 weeks of age,Group Ⅰ significantly improved the feed conversion rate compared with group Ⅱ(P＜0.05),the villus height of duodenum,jejunum and ileum was also significantly increased,and the crypt depth was significantly decreased(P＜0.05).2)The significant differences in beta diversity of the cecal microbiota between the two groups indicated that Group Ⅰ was enriched with Bifidobacterium,Actinobacteriota and Verruco microbiota,which was capable of activating lipid metabolism and enhancing the intestinal barrier,Group Ⅱ was enriched with Bacteroidota,Barnesiellaceae and Desulfobacterota,which inhibited related enzyme activity and hinder intestinal epithelial energy metabolism(P＜0.05).In summary,there are significant differences in the intestinal tissue morphology and microbial community structure of high-quality broiler chickens by environmental parameters of different types of chicken houses.Among them,the closed-loop control model is more suitable for healthy intestinal development of high-quality broilers.Maintaining a stable intestinal environment is not only of great practical value for the production challenges in intensive farming,but also provides important reference for conducting microbiome research on high-quality broiler chickens under refined regulation conditions.