METTL3、IREB2在非小细胞肺癌组织中的表达及意义OA

Objective To investigate the expression and clinical significance of methyltransferase-like protein 3(METTL3)and iron responsive element binding protein 2(IREB2)in non-small cell lung cancer(NSCLC)tissues.Methods This retrospective cohort study enrolled 102 NSCLC patients admitted to Wuxi Second People's Hospital from January 2021 to March 2023,and 100 patients with benign lung lesions served as the normal control.The expression levels of METTL3 and IREB2 in normal lung tissues,tumor tissues,and paracan-cerous tissues were detected by RT-PCR.The clinical data of all patients were collected,and the relationships of METTL3 and IREB2 expression with the clinicopathological features of NSCLC patients were analyzed.Based on the follow-up status,NSCLC patients were divided into the survival group and the death group.Univariate analysis and multivariate Cox regression analysis were conducted to analyze the factors affecting the prognosis of NSCLC patients.Receiver operating characteristic(ROC)curve was plotted to evaluate the predictive value of METTL3 and IREB2 for the prognosis in NSCLC patients.Results The expression level of METTL3 in tumor tissues of NSCLC patients was higher than that in paracancerous tissues,while the expression level of IREB2 was lower than that in paracancerous tissues,with statistically significant differences(P<0.001).Compared with patients at TNM stages Ⅰto Ⅱ or those without lymph node metastasis,the expression level of METTL3 was significantly increased and IREB2 was significantly decreased in cancer tissues of patients at stages ⅢtoⅣ or those with lymph node metastasis,with statistically significant differences(P<0.05).Multivariate Cox regression analysis results indicated that TNM stages Ⅲ to Ⅳ,lymph node metastasis,high METTL3 expression,and low IREB2 expression were all the independent risk factors for the poor prognosis in NSCLC patients(P<0.05).ROC curve analysis showed that the combined use of METTL3 and IREB2 expression in cancer tissues predicted the prognosis of NSCLC patients with an area under the curve of 0.865,a sensitivity of 89.30%,and a specificity of 78.36%,all of which were higher than those of either marker alone.Conclusion The high expression of METTL3 and low expression of IREB2 in tumor tissues of NSCLC patients are closely associated with clinicopathological features,and serve as independent risk factors for poor prognosis of patients.Furthermore,the combination of these two markers demonstrates high predictive value for poor prognosis in NSCLC patients.