基于TNF-α/NF-κB信号通路探究三橘荔核汤治疗Ⅲ型前列腺炎的机制OA

Objective It is to explore the mechanism of Sanjulihe Decoction in the treatment of typeⅢprostatitis based on tumor necrosis factor-α(TNF-α)/nuclear factor-κB(NF-κB)signaling pathway.Methods Seventy SD rats were ran-domly divided into 7 groups,with 10 rats in each group.The rats of the normal group were normally fed,while the rats of the sham operation group were injected with normal saline into ventral prostate,the rats of the model group,Preprostatitis Capsule group,low-dose,medium-dose,and high-dose groups of Sanjulihe Decoction were injected with a mixture of puri-fied rat prostate protein and Freund's complete adjuvant into ventral prostate to establish models of type Ⅲ prostatitis.Starting from the 3rd day after modeling,the low-dose,medium-dose,and high-dose groups of Sanjulihe Decoction were administered Sanjulihe Decoction 5.4 g/(kg·d),10.8 g/(kg·d),and 21.6 g/(kg·d)via gavage,respectively,the Preprostatitis Capsule group was givn aqueous solution of Preprostatitis Capsule 0.54 g/(kg·d)via gavage,while the nor-mal group,sham operation group and model group were given an equivalent volume of saline via gavage,all once daily.Af-ter 4 weeks of continuous gavage,the pathological morphology of prostate tissue was observed by HE staining,the contents of interleukin-2(IL-2),interleukin-18(IL-18),transforming growth factor-β1(TGF-β1),and cyclooxygenase-2(COX-2)in prostate tissue were determined by ELISA,the expression of proteins and mRNA related to the TNF-α/NF-κB signa-ling pathway in prostate tissue were detected by Western blot and RT-PCR methods.Results Compared with the sham opera-tion group and normal group,extensive inflammatory cell infiltration in prostate tissue was found in rats of the model group;the content of IL-2 in prostate tissue of the rats was significantly reduced(P<0.05),while the contents ofIL-18,TGF-β1 and COX-2,as well as the relative proteins and mRNA expressions of NF-κB,IKKα,TNF-α,IκBα were significantly in-creased(all P<0.05).Compared with the model group,inflammatory cell infiltration in prostate tissue was reduced in rats of all medicated groups;the contents of IL-2 in prostate tissue of the rats were significantly increased(all P<0.05),while the contents of IL-18,TGF-β1 and COX-2,as well as the relative proteins and mRNA expressions of NF-κB,TNF-α,IκBα were significantly decreased(all P<0.05).Compared with Preprostatitis Capsule group,the contents of IL-2 in prostate tissue were more higher(both P<0.05),while the contents ofIL-18,TGF-β1 and COX-2,as well as relative protein and mRNA expressions of NF-κB,IKKα and IκBα in prostate tissue were more lower in rats of the medium-dose,and high-dose groups of Sanjulihe Decoction(all P<0.05).Conclusion Sanjulihe Decoction can treat type Ⅲ prostatitis possibly via regulating TNF-α/NF-κB signaling pathway.