妇康栓对产后大鼠子宫复旧的影响研究OA

Objective:It is to investigate the regulatory effect of Fukang Suppository on uterine involution in postpartum rats.Methods Fifty pregnant SD rats were randomly divided into model group,Carboprost Suppository group,and low-dose,medium-dose,and high-dose groups of Fukang Suppository,with 10 rats in each group,another 10 non-pregnant fe-male rats were taken as the blank group.Starting from the 7th day after delivery,the Carboprost Suppository group was giv-en Carboprost Suppository 0.105 mg/kg by vaginal administration,the low-dose,medium-dose and high-dose groups of Fu-kang Suppository were given Fukang Suppository 0.262 g/kg,0.525 g/kg,and 1.05 g/kg by vaginal administration,re-spectively,all administrated once daily.After 14 days of continuous treatment,the histopathology of uterine tissue was ob-served by HE staining;the serum levels of sex hormones[estradiol(E2),progesterone(P)],inflammatory cytokines[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10)],immunoglobulins[immunoglobulin G(IgG),immunoglobulin A(IgA)],and growth factors[transforming growth factor-β1(TGF-β1),transforming growth fac-tor-β3(TGF-β3),vascular endothelial growth factor(VEGF)]were measured by ELISA;the protein expressions of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in uterine tissue were detected by Western blot analysis.Results Compared with the blank group,the uterine cavity morphology of rats in the model group was abnormal,with thinner endometrium and partial discontinuity of endometrial continuity;the serum levels of E2,IL-10,TGF-β3,and VEGF,as well as the TGF-β3/TGF-β1 ratio in the rats were significantly reduced(all P<0.05),while the serum levels of P,TNF-α,IL-6,IgA,IgG and TGF-β1 were significantly increased(all P<0.05);the relative protein expression of MMP-9 in uterine tissue was significantly increased(P<0.05),while the relative protein expression of TIMP-1 was significantly reduced(P<0.05).Compared with the model group,the uterine cavity morphology of rats in each medicated group was approaching normal,with better endometrial continuity;the serum levels of E2,P(except for the Carboprost Suppository group),IL-10,TGF-β3,and VEGF(except for the Carboprost Suppository group),as well as the TGF-β3/TGF-β1 ratio in rats of the Carboprost Suppository group and high-dose group of Fukang Suppository were sig-nificantly increased(all P<0.05),while the serum levels ofTNF-α,IL-6,IgG and TGF-β1 were significantly decreased(all P<0.05);the relative protein expression of MMP-9 in uterine tissue was significantly decreased(P<0.05),while the relative protein expression of TIMP-1 was significantly increased(P<0.05).Conclusion Fukang Suppository can alle-viate inflammatory response,regulate the levels of sex hormones and growth factors and balance of matrix metalloproteinase family and their inhibitors,promote the remodeling of uterine matrix,and contribute to uterine involution in postpartum rats.