"迎香"穴位注射通过调控Treg/Th17免疫平衡缓解变应性鼻炎鼻部过敏症状的机制OA
"Yingxiang"(LI2)acupoint injection alleviates nasal allergy symptoms in allergic rhinitis rats by regulating Treg/Th17 immune balance
目的:观察"迎香"穴位注射对变应性鼻炎(AR)大鼠辅助性T细胞 17(Th17)、调节性T细胞(Treg)及相关细胞因子表达的影响,探讨"迎香"穴位注射治疗AR的作用机制.方法:24只SD大鼠随机分为正常组、模型组和穴位注射组,每组 8只.采用卵清蛋白致敏法建立AR大鼠模型.穴位注射组于双侧"迎香"穴位注射地塞米松和利多卡因混合液,每3 d干预1次,共4次.通过行为学观察各组大鼠鼻部过敏症状并进行评分;HE染色法观察大鼠鼻黏膜组织形态;流式细胞术检测外周血Th17、Treg百分比;ELISA法检测血清过敏原特异性免疫球蛋白E(sIgE)、白细胞介素(IL)-10、IL-17A的含量;实时荧光定量PCR法和Western blot法分别检测鼻黏膜叉头状转录因子p3(Foxp3)、维甲酸孤儿受体γt(RORγt)mRNA和蛋白表达水平.结果:与正常组比较,模型组大鼠鼻部肿胀暗红,鼻周可见抓挠痕迹,且伴随明显血污及血痂;症状评分、外周血Th17百分比、血清sIgE和IL-17A含量、鼻黏膜RORγt mRNA及蛋白表达水平升高(P<0.01),而外周血Treg百分比、血清IL-10含量、鼻黏膜Foxp3 mRNA及蛋白表达降低(P<0.01).与模型组比较,穴位注射组大鼠鼻部外观及颜色基本恢复正常,未见明显挠痕、血污及血痂;症状评分、外周血Th17百分比、血清sIgE和IL-17A含量、鼻黏膜RORγt mRNA及蛋白表达水平均降低(P<0.01),而外周血Treg百分比、血清IL-10含量、鼻黏膜Foxp3 mRNA及蛋白表达升高(P<0.01).HE染色结果显示,模型组大鼠鼻黏膜层增厚,黏膜上皮细胞排列欠规则,部分纤毛结构缺失,黏膜层及固有层可见大量炎性细胞浸润;穴位注射组大鼠鼻黏膜组织结构清晰,黏膜上皮细胞排列规则,纤毛数量丰富,仅见零散炎性细胞浸润.结论:"迎香"穴位注射可有效改善AR大鼠的鼻部过敏症状,其作用机制可能与调节Treg/Th17免疫平衡、抑制炎性反应有关.
Objective To observe the effect of"Yingxiang"(LI2)acupoint injection on T-helper 17(Th17)cell,regulatory T(Treg)cell,and related cytokine expressions in allergic rhinitis(AR)rats,so as to explore its possible mechanism in improving AR.Methods Twenty-four SD rats were randomly divided into normal,model,and acupoint injection groups(n=8).The AR model was established using ovalbumin sensitization.The rats of acupoint injection group received injection of a mixture solution of dexamethasone and lidocaine(0.05 mL/acupoint)at bilateral LI2,once every 3 d for a total of 4 times.Nasal allergy symptom scores were recorded;histopathological changes in the nasal mucosa were observed via HE staining;flow cytometry was used to detect the percentage of Th17 and Treg in peripheral blood;the levels of serum allergen-specific immunoglobulin E(sIgE),interleukin(IL)-10 and IL-17A were detected by ELISA;the mRNA and protein expression levels of forkhead transcription factor p3(Foxp3)and retinoic acid orphan receptor γt(RORγt)in nasal mucosa were detected by real-time fluorescence quantitative PCR and Western blot,respectively.Results Following modeling,the symptom scores,Th17/CD4+T cell ratio in peripheral blood,serum contents of sIgE and IL-17A,and RORγt mRNA and protein expression levels in nasal mucosa were significantly increased(P<0.01),while the Treg/CD4+T cell ratio,serum IL-10 content,and Foxp3 mRNA and protein expression levels in nasal mucosa were significantly decreased(P<0.01).After acupoint injection intervention,the modeling induced increase and decrease of indexes mentioned above were completely reversed(P<0.01).HE staining revealed thickened nasal mucosa,disordered epithelial arrangement,partial cilia loss,and massive inflammatory cell infiltration in the model group,which were markedly improved in the acupoint injection group.Conclusion"Yingxiang"acupoint injection can effectively alleviate nasal allergy symptoms in AR rats,which may be related to its function in regulating Treg/Th17 immune balance and suppressing inflammatory responses.
卢晓晓;石佳惠;侯珣瑞;梁欣;李丽红
贵州医科大学附属医院,贵阳 550004贵州医科大学附属医院,贵阳 550004贵州医科大学附属医院,贵阳 550004贵州医科大学附属医院,贵阳 550004贵州医科大学附属医院,贵阳 550004
变应性鼻炎穴位注射Treg/Th17免疫平衡细胞因子迎香
Allergic rhinitisAcupoint injectionTreg/Th17 immune balanceCytokineYingxiang(LI2)
《针刺研究》 2026 (3)
351-357,7
国家自然科学基金项目(No.82360891)贵州医科大学国家自然科学基金培育项目(No.19NSP049)
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