黄芪建中汤合小承气汤调控术后肠梗阻大鼠肌层巨噬细胞极化对Cajal间质细胞的影响OA

Objective It is to investigate the mechanism of Huangqi Jianzhong Decoction combined with Xiaochengqi Decoction(HQJZ-XCQ)in affecting interstitial cells of Cajal(ICC)in rats with postoperative ileus(POI)via regulating muscularis macrophage(MMs)polarization.Methods Twenty-four SPF male Wistar rats were randomly divided into 4r groups:normal group,model group,soap solution group and HQJZ-XCQ group,with 6 rats in each group.The rats of all groups except for the normal group were prepared with intestinal surface abrasion method to establish models of POI.Start-ing from the first postoperative day,the HQJZ-XCQ group was given HQJZ-XCQ group 8.45 g/(kg·d)by enema,the soap solution group was given an equal volume of soap solution by enema,both administered once daily,continuously trea-ted for one week.At the end of treatment,the small intestinal propulsion rate was measured,the contents of MMC surface markers CD86 and CD206,as well as inflammatory factors tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)in small intestinal tissue were determined by ELISA,the protein expression of the ICC marker c-Kit protein in small intestinal tissue was detected by Western blot and immunofluorescence assays.Results Compared with the normal group,obvious e-dema,adhesions,and inflammatory infiltration were found in intestinal wall of rats in the model group;the small intestinal propulsion rate of the rats was significantly reduced(P<0.05);the contents of CD86,CD206 and TNF-α in small intesti-nal tissue were significantly increased(all P<0.05),while the content of IL-10,the relative protein expression of c-Kit,and the mean optical density value of positive protein expression of c-Kit were significantly reduced(all P<0.05).Com-pared with the model group,the edema,adhesions,and inflammatory infiltration in intestinal wall of rats in the soap solu-tion group and HQJZ-XCQ group were alleviated;the small intestinal propulsion rate of rats in the HQJZ-XCQ group was significantly increased(P<0.05),the contents of CD206 and TNF-α in small intestinal tissue were significantly decreased(all P<0.05),while the content of IL-10,the relative protein expression of c-Kit,and the mean optical density value of positive protein expression of c-Kit were significantly increased(all P<0.05);the contents of CD86,CD206 and TNF-α in small intestinal tissue of rats in the soap solution group were significantly decreased(all P<0.05),while the content of IL-10 was significantly increased(P<0.05).The small intestinal propulsion rate and the contents of CD86,CD206 and IL-10 in small intestinal tissue of rats in the HQJZ-XCQ group were significantly higher than those in the soap solution group.Conclusion Huangqi Jianzhong Decoction combined with Xiaochengqi Decoction can obviously alleviate intestinal injury and promote motility recovery in POI rats.The mechanism may involve the modulation of MMs polarization towards the M2 phenotype,inhibition of inflammatory responses,and reduction of ICC apoptosis.