正交实验法考察高良姜-香附药对胃肠运动调节作用OA

Objective To explore the effect of different proportions of Alpinia officinarum and Cyperus rotundus compat-ibility on gastrointestinal motility based on orthogonal design,and to study the compatibility law of Alpinia officinarum and Cyperus rotundus,so as to provide a scientific basis for the clinical application of the drug pair.Methods Based on the classic formula design of Alpinia Officinarum and Cyperus rotundus,different ratios and doses were tested based on orthogonal experimental de-sign.By monitoring the defecation and bead expulsion of normal and constipated mice,as well as the small intestine propulsion rate and gastric emptying rate,the analytic hierarchy process combined with criteria importance through intercriteria correlation(AHP-CRITIC)method was used to comprehensively evaluate the effects of different combinations.The optimal combination ratio and dose were selected based on the orthogonal design method.Results Different combinations of Alpinia officinarum and Cyperus rotundus exhibited distinct effects.Using the AHP-CRITIC method,it was found that in terms of regulating gastrointestinal motili-ty,Alpinia officinarum was more significant than Cyperus rotundus.The top three combinations were Alpinia officinarum and Cype-rus rotundus 2:1(5.4 g·kg-1)group,Alpinia officinarum(5.4 g·kg-1)group,and Alpinia officinarum and Cyperus rotundus 1:1(10.8 g·kg-1)group.Their comprehensive scores are 81.21,80.64 and 78.54,respectively.Conclusion The experi-ments proved that the optimal ratio of Alpinia officinarum and Cyperus rotundus is 2:1,which provides a reference for further in-depth research and clinical application of this herb couples.