四君子汤对结肠病变小鼠模型的作用机制OA

Objective To investigate the therapeutic effects of Sijunzi decoction on dextran sulfate sodium(DSS)-in-duced colonic lesions in mice.Methods The aqueous extract of Sijunzi decoction was prepared via water decoction,and its pol-ysaccharides were isolated using ethanol precipitation and the Sevag method.The purity,molecular weight,and monosaccharide composition of the polysaccharides were analyzed via high-performance liquid gel chromatography and gas chromatography-mass spectrometry(GC-MS).Mice were divided into normal,model,positive drug control(mesalazine,0.1 g·kg-1),aqueous extract(5 g·kg-1 and 15 g·kg-1,based on raw herb weight),and polysaccharide(0.18 g·kg-1 and 0.53 g·kg-1,equivalent to raw herb doses of 5 g·kg-1 and 15 g·kg-1)groups.Except for the normal group,modeling mice were administered 3％DSS in drink-ing water for 7 days to induce colitis(with concurrent oral treatments).Disease Activity Index(DAI)scores were assessed on day 4.On day 8,animals were anesthetized and sacrificed,and their colon length,mass,mass-to-length ratio,macroscopic and his-topathological injury scores were measured.Immunohistochemistry was used to evaluate colonic protein expression levels.Results Sijunzi decoction polysaccharides were heteropolysaccharides with a relative molecular mass of approximately 4881 Da,com-posed of glucose(79.06％),galactose(9.49％),arabinose(7.37％),and minor monosaccharides.Both the aqueous extract and polysaccharides reduced DAI scores,macroscopic and histopathological damage,alleviated colon shortening and weight gain,and restored the decreased expression of colonic HuR,E-cadherin,Rac1,and Cdc42 proteins induced by DSS.Conclusion The aqueous extract and polysaccharides of Sijunzi decoction exert protective effects against DSS-induced colonic lesions in mice,likely mediated by upregulating colonic HuR,E-cadherin,Rac1,and Cdc42 protein expression.