固相萃取-高效液相色谱法测定盐附子中乌头碱含量及大鼠血浆附子类生物碱浓度OA

Objective To establish a solid phase extraction-high performance liquid chromatography(SPE-HPLC)method for the rapid determination of aconitine,mesaconitine,hypaconitine,lappaconitine,and benzoylaconine in salt-processed Aconiti Lateralis Radix Praeparata(Yan Fuzi)and rat plasma 30 min after intragastric administration of a Yan Fuzi decoction.Methods Zeolitic imidazolate framework-8(ZIF-8)material was synthesized and characterized according to literature methods.By optimizing the preparation process of metal-organic frameworks and solid-phase extraction conditions,a quantitative analytical method coupling extraction with liquid chromatography was developed.This method was applied to detect the plasma concentrations of aconitine,hypaconitine,mesaconitine,lappaconitine,and benzoylaconine in rat plasma following intragastric administration of Yan Fuzi decoction(0.375 g·kg-1 body weight).Results The contents of five aconite alkaloids in Yan Fuzi were determined as follows:aconitine 62.78 mg·kg-1,mesaconitine 500.4 mg·kg-1,hypaconitine 37.80 mg·kg-1,lappaconitine 0.144 mg·kg-1,and benzoylaconine 36.14 mg·kg-1.In plasma samples collected 30 min post-administration,the concentra-tions detected were:aconitine 2.06 μg·mL-1,mesaconitine 11.79 μg·mL-1,hypaconitine 1.69 μg·mL-1,lappaconitine 1.77 μg·mL-1,and benzoylaconine 23.22 μg·mL-1.Method validation confirmed that all parameters met quantitative determi-nation requirements.Conclusion The established method enables accurate quantification of aconite alkaloids in both Aconiti Lateralis Radix Praeparata and plasma samples after oral administration,providing a valuable reference for clinical monitoring of these compounds in biological matrices.