鳖甲活性肽抗肝癌作用网络药理学探讨及体外活性验证OA

Objective Anti-hepatocellular carcinoma mechanism of active peptides derived from hydrolyzates of turtle shell protein(HTSP)was elucidated by employing network pharmacology,molecular docking,and in vitro assays.Methods The study collected two active peptides from the hydrolysates of turtle shell protein(HTSP),namely HTSP-9 and HTSP-11,which were previously identified by our research group.The potential targets of these active peptides were predicted using the SuperPred and Swiss Target Prediction websites.Subsequently,the HCC-related targets were screened through databases including Gene-Cards,DisGeNET,and OMIM to identify the overlapping targets between the two peptides.Subsequently,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were conducted on the overlapping targets.Cy-toscape software was used to construct the HTSP-HCC-Overlapping Targets protein-protein interaction network(PPI)and screen core targets.Molecular docking experiments were performed on the HTSP and the core targets of action using AutoDock software,and finally,the network pharmacology findings were corroborated by in vitro cellular assays.Results The analysis yielded 37 core targets shared by the HTSP and HCC.The KEGG enrichment analysis involves signaling pathways such as PI3K-Akt,TNF,and apoptosis.In vitro experiments demonstrated that the HTSP could inhibit the proliferation of HepG2 cell.Conclusion The HTSPs are likely to exert therapeutic effects against HCC by modulating the growth and differentiation of hepatocellular carcinoma HepG2 cells through a multi-target and multi-pathway approach.