余甘子转录组与SSR信息分析及分子标记开发OA

[Objective]This research aims to clarify the distribution characteristics of SSR loci in the transcriptome of Phyllanthus emblica and develop the SSR molecular marker technology of P.emblica,to provide a theoretical basis for the identification of genetic relationships,genetic breeding,and the innovative utilization of P.emblica.[Method]Six leaf samples of P.emblica exhibiting significant phenotypic diffe-rences were utilized as materials.Non-parametric transcriptome data for these leaves were obtained through high-throughput sequencing technology,followed by an analysis of the transcriptome and SSR locus chara-cteristics.SSR primers were then designed and evaluated for their effectiveness and polymorphism.[Re-sult]The results indicated that a total of 65 074 genes were identified through transcriptome sequencing of P.emblica leaves,with an average length of 1 064 bp.Among them,71.77%of the genes were successfully annotated.Additionally,a total of 18 245 SSR loci were identified across all genes,showing a frequency of 21.68%,which included 192 repeat motifs,with A/T repeat motifs being the most abundant.Among all lo-ci,2 714 polymorphic SSRs were detected,accounting for 14.88%,suggesting that SSR loci had great po-tential for genetic diversity analysis.A total of 12 488 pairs of SSR primers were su-ccessfully designed for the 18 245 genes containing SSR loci,with a success rate of 73.88%.Among all the primers,7 602 pairs of primers had the repeat motif of mononucleotide,accounting for 60.87%.Subsequently,154 pairs of primers were randomly selected for validation and polymorphism analysis.Ultimately,10 pairs of SSR primers ex-hibiting clear amplified bands and good polymorphism were identified.The repeat motifs of these 10 pairs consisted of 7 dinucleotides,1 trinucleotide,and 2 pentanucleotides.The size of the amplified products ranged from 127 to 270 bp,with an average of 220.5 bp.[Conclusion]Polymorphic SSR loci hold signifi-cant potential for genetic diversity.10 pairs of SSR primers,which exhibited clear amplified bands and sub-stantial polymorphism were selected.