桃胶缓解重金属所致皮肤成纤维细胞损伤的作用研究OA

A heavy metal-induced human skin fibroblast(HFF-1)damage model was established using nickel sulfate(NiSO4).The protective effects of peach gum against heavy metal-induced cytotoxicity were evaluated through cell viability assays,morphological observation,and DNA damage marker(γ-H2AX)detection.Results demonstrated that exposure to NiSO4(8 mmol/L)for 12 h reduced HFF-1 cell viability to 44.65%(p<0.01)and significantly increased γ-H2AX expression in cells by 27.16-fold compared with the control.Pre-treatment with 8 mg/mL peach gum significantly improved cell viability to 59.22%(p<0.01)and decreased γ-H2AX expression to 3.8-fold of the control,indicating substantial protection against DNA damage.Component analysis revealed that the total sugar content of peach gum was 43.8%(phenol-sulfuric acid method).The polysaccharide fraction had an average molecular weight of 803.81 kDa(determined by high-performance liquid gel permeation chromatography)and was primarily composed of arabinose(47.96%),galactose(33.16%),xylose(13.41%),mannose(3.52%),and rhamnose(1.95%)as determined by gas chromatography-mass spectrometry(GC-MS).These findings suggest that the polysaccharide components in peach gum may alleviate heavy metal-induced cytotoxicity in skin fibroblasts,potentially through the mitigation of DNA damage mechanisms.