10种花类提取物的活性评价及在化妆品中的应用OA

To screen floral materials with significant whitening activity and optimize their synergistic ratio for the cosmetic applications,their cosmetic application values have been evaluated.Ten floral extracts were initially screened using DPPH radical scavenging assay and tyrosinase activity inhibition test.The three most active ingredients were selected for the ratio optimization via response surface methodology.The efficacy of the optimized combination was evaluated through B16F10 cell experiments.A lotion containing 0.1％of the optimized combination was formulated for a 28-day human efficacy study(measuring ITA° value and melanin index)and safety assessment.Peony,roselle,and crape myrtle flowers demonstrated that they all have superior antioxidant activity,with the optimal ratio being 1:2:1.9.At this ratio,the 0.1％combination significantly inhibited melanin synthesis(34.67％)and tyrosinase activity(34.99％),while maintained the cell viability(84.30％).Human trials showed a significant increase in ITA° value(+4.45％,p<0.001)and a reduction in melanin content(-14.72％,p<0.001)with good safety profile.The 1:2:1.9 combination of peony flower,roselle flower,and crape myrtle flower exhibits remarkable skin-whitening efficacy,which is suitable for the cosmetic development.