富含原花青素的红山茶花提取液制备及其功效研究OA

Using Zhejiang red camellia(Camellia sinensis)as the raw material,the purification process for proanthocyanidins was optimized using D101 macroporous resin.A proanthocyanidin-rich red camellia extract was prepared,and its functional activities were systematically evaluated.The results showed that purification with D101 macroporous resin increased the proanthocyanidin purity to 40.69％,representing a 9.64-fold enhancement compared with the unpurified extract.In vitro activity assays demonstrated that the proanthocyanidin-rich Camellia sinensis extract exhibited strong antioxidant capacity,effectively scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals with an IC50 value of 0.19％.At a concentration of 5％,the extract inhibited the formation of advanced glycation end products(AGEs)by 47.81％±1.59％.At 0.05％,the extract significantly promoted wound healing in human immortalized keratinocytes,achieving a healing rate of 63.99％±3.89％.Furthermore,treatment with 0.30％extract markedly increased the relative levels of type Ⅰ and type Ⅲ collagen in human normal fibroblasts by 71.75％±3.55％and 27.80％±1.49％,respectively,while reducing matrix metalloproteinase-1(MMP-1)expression by 63.38％±2.80％.These effects were mediated through activation of the TGF-β1/Smads signaling pathway.In conclusion,the proanthocyanidin-rich Camellia sinensis extract exhibits potent antioxidant,anti-glycation,skin repair,and anti-wrinkle activities,highlighting its potential application in functional cosmetics and skin care formulations.