新风胶囊调节PI3K/AKT信号通路减轻骨关节炎大鼠炎症及骨代谢紊乱的机制研究OA

Objective To investigate the effects of Xinfeng Capsule(新风胶囊,XFC)on inflammation and bone metabolism in osteoar-thritis(OA)rats via the PI3K/AKT signaling pathway.Methods Sixty-four SD rats were randomly divided into eight groups:the Control group,Model group,low/medium/high-dose XFC(XFC-L,XFC-M,XFC-H)groups,celecoxib group,AKT activator SC79 group,and XFC-H+SC79 group.The OA Model was induced in all groups except the Control group by intra-articular injection of collagenase.Cartilage histology was analyzed using Safranin O/Fast Green and Alcian blue staining.Serum levels of TGF-β1,TNF-α,IL-1β,IL-6,TRACP-5b,IGF-1,OPG,and BALP were measured by ELISA.The protein expression levels of PI3K,p-PI3K,AKT,and p-AKT in cartilage were detected by Western blot(WB)analysis.Results Compared with the Control group,the Model group presented with a rough cartilaginous surface,reduced chondrocytes and extracellular matrix,and a destroyed tidemark.The expression levels of IL-1β,IL-6,TNF-α,TGF-β1,TRACP-5b,p-PI3K,and p-AKT were significantly elevated(P<0.05),whereas the expression levels of OPG,BALP,and IGF-1 were significantly reduced(P<0.05).Compared with the Model group,XFC treatment resulted in reduced expression levels of IL-1β,IL-6,TNF-α,TGF-β1,TRACP-5b,p-PI3K,and p-AKT(P<0.05),increased expression levels of OPG,BALP,and IGF-1(P<0.05),and alleviated cartilage damage.The SC79 group exhibited more severe inflammation,bone metabolism disorders,and cartilage damage compared with the Model group.Compared with the XFC-H group,co-administration of SC79 partially reversed the ameliorative effects of XFC-H on inflammation,bone metabolism disorder,and cartilage damage.Conclu-sion XFC alleviates inflammation and bone metabolism disorders in OA rats and ameliorates cartilage damage by regulating the PI3K/AKT signaling pathway.