载木犀草素PMMA骨水泥对桡骨缺损大鼠成骨修复的影响OA

Objective:To explore the effect of polymethyl methacrylate(PMMA)bone cement loaded with lute-olin on osteogenic repair in rats with radial defects.Methods:The rat model of radius defect was constructed and di-vided into control group,PMMA bone cement group and PMMA bone cement+luteolin group,with 18 rats in each group.No material was implanted in the bone defect of the control group;PMMA bone cement group rats were im-planted with PMMA bone cement without loaded luteolin at the bone defect site;PMMA bone cement+luteolin group was implanted with PMMA bone cement loaded with luteolin.At the fifth week after modeling,autologous cancellous bone was implanted at the incision of the original radius in 6 rats from each group.The general state of the rats was observed during the experiment;X-ray was used to observe the contour of bone cement,the reaction range of bone cement and surrounding tissue,and bone healing in the bone defect area;hematoxylin-eosin staining was used to observe the morphological changes of induces membrane and newly formed bone in bone defect areas,and the propor-tion of new bone and the number of osteoblasts were evaluated;the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in induces membrane tissue were detected by enzyme-linked immunosorbent assay;the expressions of vascular endothelial growth factor(VEGF),transforming growth faction-β1(TGF-β1)and bone morphogenetic protein-2(BMP-2)in induces membrane tissue were detected by western blotting.Results:During the experiment,all animals had good appetite,physical activity,and no deaths;One week after surgery,edema,congestion,swelling,and pain were observed in all surgical sites in each group.At the 5th week after modeling,compared with control group,the bone cement contour in the bone defect area of the PMMA bone cement group became irregular,and the interface between the bone cement and the surrounding tissue appeared transparent area,and the low-density soft tissue shad-ow could be seen around it,many capillary endothelial cells in the induced membrane were disordered,and the levels of IL-6 and TNF-α in the induces membrane tissue were significantly reduced,the protein expressions of VEGF,TGF-β1 and BMP-2 were significantly increased(all P<0.05).Compared with PMMA bone cement group,the bone cement shape irregularity and soft tissue shadow around the bone defect area in the PMMA bone cement+luteolin group were more significant,and the induced membranes contained a large number of capillary endothelial cells ar-ranged neatly,and the levels of IL-6 and TNF-α in the induces membrane tissue were significantly reduced,the pro-tein expressions of VEGF,TGF-β1 and BMP-2 were significantly increased(all P<0.05).At the 7th week after bone grafting,uniformly stained new bone tissue was found in the bone defect area of the 3 groups,and the new bone con-tained osteoblasts and bone matrix;compared with control group,the PMMA bone cement group showed obvious bone bridging in the bone defect area,and the signs of bone healing were significantly improved,Lane-Sandhu X-ray score,the proportion of new bone and osteoblast number were significantly increased(all P<0.05).Compared with PMMA bone cement group,the bone bridge in the bone defect area of the PMMA bone cement+luteolin group was more continuous and mature,the bone defect gap was significantly reduced,the bone healing process was more rapid and complete,and the Lan-Sandhu X-ray score,the proportion of new bone and osteoblast number were significantly increased(all P<0.05).Conclusion:PMMA bone cement loaded with luteolin can synergistically promote new bone formation and bone healing by in-hibiting inflammation and enhancing the osteogenic activity of induced membrane in rats with radius defects.