葛根提取物通过基质细胞衍生因子-1调控正畸大鼠牙周膜干细胞增殖和成骨分化的研究OA

Objective To investigate whether Pueraria lobata extract(PLE)affects the proliferation and osteogenic differentiation of periodontal ligament stem cells(PDLSCs)under orthodontic static pressure by modulating stromal cell-derived factor-1(SDF-1)expres-sion.Methods PDLSCs were isolated from the periodontal ligament of the first molars of SD rats.Third-passage PDLSCs were subjected to orthodontic static pressure(OSP)and divided into the following groups:control(NC,no treatment),OSP,PLE-L(OSP+25 µg/mL PLE for 24 h),PLE-H(OSP+50 µg/mL PLE for 24 h),AMD3100(OSP+20 µmol/L SDF-1 inhibitor AMD3100 for 24 h),and PLE-H+AMD3100(OSP+50 µg/mL PLE and 20 µmol/L AMD3100 for 24 h).Cell proliferation was assessed using the CCK-8 assay.Alka-line phosphatase(ALP)activity was measured by colorimetry,and mineralization was evaluated by Alizarin red staining.The protein levels of proliferating cell nuclear antigen(PCNA),osteocalcin(OCN),osteopontin(OPN),RUNX family transcription factor 2(Runx2),and SDF-1 were determined by Western blot(WB)analysis.Results Compared with the NC group,the OSP group showed significant increases in OD450 values,ALP activity,mineralized nodule formation,and the protein expression levels of PCNA,OCN,OPN,Runx2,and SDF-1(all P<0.05).Compared with the OSP group,the PLE-L/H groups exhibited further enhancements in these same parameters(all P<0.05).In contrast,the AMD3100 group showed decreases in all measured outcomes compared to the OSP group(P<0.001).Furthermore,AMD3100 treatment attenuated the promoting effects of high-dose PLE on PDLSC proliferation and osteogenic differentiation.Conclusion PLE may promote the proliferation and osteogenic differentiation of PDLSCs in rats stimulated by orthodontic static pressure through up-regulation of SDF-1.