层次分析法‑熵权法结合Box‑Behnken响应面法优化加味桔梗汤水提醇沉工艺OA

Objective To establish a validated ultra-performance liquid chromatography(UPLC)method for the simultaneous quantification of multiple components in modified Jiegeng Decoction and to optimize its water extraction and ethanol precipitation processes using the Box-Behnken design-response surface method(BBD-RSM).Methods Platycodin D was determined by UPLC coupled with evaporative light scattering detection(UPLC-ELSD),while gallic acid,chlorogenic acid,corilagin,hesperidin,and rosmarinic acid were quantified via UPLC with ultraviolet detection(UPLC-UV).Weight coefficients for each evaluation index were calculated using the analytic hierarchy process(AHP)-entropy weight method.Based on the results of single-factor experiments,BBD-RSM was employed to investigate the effects of soaking time and decoction time on the water extraction process,as well as the impacts of ethanol concentration,precipitation time,and extract density on the ethanol precipitation process.Results A UPLC method for the simultaneous determination of multiple components in modified Jiegeng Decoction was successfully established and validated.The optimized water extraction conditions were confirmed as follows:no soaking,12-fold volume of water relative to the raw material,and two rounds of decoction(65 minutes per round).For ethanol precipitation,the optimal parameters were determined to be 80%(V/V)ethanol concentration,48 hours of precipitation,and an extract density of 1.65 g·mL-1.Conclusions The established UPLC method exhibits high accuracy and reliability,and the optimized extraction-precipitation parameters demonstrate good stability.This study provides a solid technical foundation for subsequent pharmacological research and large-scale industrial production of Modified Jiegeng Decoction.