黑果枸杞+肉苁蓉调节PI3K/Akt信号通路对老年性骨质疏松大鼠影响的研究OA
Research on the effect of Fructus Lycii Ruthenic and Herba Cistanches on senile osteoporosis in aged rats via regulation of the PI3K/Akt signaling pathway
目的 探讨黑果枸杞+肉苁蓉通过调控磷脂酰肌醇3-激酶(PI3K)/丝氨酸-苏氨酸激酶(Akt)信号通路对老年性骨质疏松症(SOP)大鼠的影响.方法 48只SD大鼠随机分为空白组、衰老组、VE组(0.018 g·kg-1)、PI3K激活剂组(740 Y-P组,10 mg·kg-1)、黑果枸杞+肉苁蓉组(1 440 mg·kg-1)、黑果枸杞+肉苁蓉+740 Y-P组.Micro-CT检测大鼠骨微结构变化;HE染色观察股骨病理形态、碱性磷酸酶钙-钴染色检测大鼠股骨中成骨细胞活性、抗酒石酸酸性磷酸酶(TRAP)染色检测大鼠股骨组织中破骨细胞活性;ELISA法检测血清肿瘤坏死因子-α(TNF-α)、骨碱性磷酸酶(BALP)、抗酒石酸酸性磷酸酶5b(TRACP-5b)含量;Western blot检测股骨组织PI3K/Akt通路蛋白表达.结果 与空白组比较,衰老组大鼠股骨微结构明显破坏,BMD、BV/TV、Tb.N、Tb.Th显著下降(P<0.05),Tb.Sp、SMI上升(P<0.05),骨组织病理性损伤明显,血清中TNF-α、TRACP-5b 含量升高,BALP含量降低(P<0.05),PI3K、Akt蛋白表达量增高(P<0.05);与衰老组比较,黑果枸杞+肉苁蓉组能够改善骨微结构,提升衰老大鼠股骨的BMD和 BV/TV、Tb.N、Tb.Th,降低 Tb.Sp、SMI(P<0.05),显著减轻衰老大鼠骨组织病理性损伤;与黑果枸杞+肉苁蓉组相比,740 Y-P组成相反趋势.结论 黑果枸杞+肉苁蓉可通过调控PI3K/Akt通路改善SOP.
Objective To investigate the effect of the medicinal couplet of Fructus Lycii Ruthenic and Herba Cistanches(FLRHC)on senile osteoporosis(SOP)in aged rats through regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)signaling pathway.Methods Forty-eight SD rats were randomly divided into a blank group,an aging model group,a vitamin E(VE)group(0.018 g·kg⁻1),a PI3K activator(740 Y-P)group(10 mg·kg⁻1),a FLRHC group(1440 mg·kg⁻1),and a FLRHC+740 Y-P group.Micro-CT was used to analyze bone microstructure.HE staining was performed to observe femoral histopathology.Alkaline phosphatase calcium-cobalt staining and tartrate-resistant acid phosphatase(TRAP)staining were used to detect osteoblast and osteoclast activity,respectively.Serum levels of tumor necrosis factor-α(TNF-α),bone alkaline phosphatase(BALP),and tartrate-resistant acid phos-phatase 5b(TRACP-5b)were measured by ELISA.The protein expression of PI3K and Akt in femoral tissue was determined by West-ern blot(WB).Results Compared with the blank group,rats in the aging model group exhibited impaired femoral microstructure,as evi-denced by significantly decreased bone mineral density(BMD),bone volume/total volume(BV/TV),trabecular number(Tb.N),and trabecular thickness(Tb.Th),along with increased trabecular separation(Tb.Sp)and structure model index(SMI)(all P<0.05).Pathological bone damage was obvious,serum TNF-α and TRACP-5b levels were elevated,BALP level was reduced,and the protein expression of PI3K and Akt was increased(all P<0.05).Compared with the aging model group,the FLRHC treatment ameliorated bone microstructure,increased BMD,BV/TV,Tb.N,and Tb.Th,decreased Tb.Sp and SMI,alleviated pathological damage,modulated the serum biomarkers,and down-regulated the expression of PI3K and Akt proteins(all P<0.05).However,the effects of FLRHC were significantly antagonized by co-administration with the PI3K activator 740 Y-P.Conclusion The medicinal couplet of FLRHC may alle-viate senile osteoporosis in aged rats,potentially through regulating the PI3K/Akt signaling pathway.
冯鹏;陈元欢;许小敏;易颖;唐欣月;李菲;孔东升;田永衍;葛生虎;张杰昌
河西学院医学院,甘肃 张掖 734000甘肃中医药大学,甘肃 兰州 730000河西学院医学院,甘肃 张掖 734000河西学院医学院,甘肃 张掖 734000河西学院医学院,甘肃 张掖 734000河西学院医学院,甘肃 张掖 734000河西学院甘肃省黑果枸杞保护及开发利用2011协同创新中心,甘肃 张掖 734000河西学院丝绸之路中医药研究中心,甘肃 张掖 734000河西学院医学院,甘肃 张掖 734000河西学院医学院,甘肃 张掖 734000
医药卫生
老年性骨质疏松PI3K/Akt信号通路黑果枸杞肉苁蓉
Senile osteoporosisPI3K/Akt signaling pathwayFructus Lycii RuthenicHerba Cistanches
《时珍国医国药》 2026 (4)
641-648,8
河西学院校长基金创新团队项目(CXTD004)甘肃省高等学校产业支撑项目(2021CYZC-56)甘肃省高校教师创新基金项目(2023B-168)大学生创新创业训练计划项目(202410740032)
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