玉屏风散加味方的胚胎毒性评估及多模态炎症调控作用研究OA

Objective To evaluate the embryotoxicity and multimodal anti-inflammatory effects of Jiawei Yupinfeng Powder(玉屏风散加味方,JYP)using a zebrafish model.Methods Zebrafish embryos were continuously exposed to JYP at concentrations ranging from 50 to 1600 µg/mL to monitor its acute toxicity on 14 endpoints,including embryonic development,cardiac function,liver metabolism,and renal excretion.Multimodal inflammation models induced by CuSO4,tail transection,and lipopolysaccharide(LPS)were established.The effects of JYP on neutrophil infiltration,nitric oxide(NO)levels,and the mRNA expression of key inflammatory factors(NLRP3,Caspase-1,IL-1β,TNF-α,IL-4)were then analyzed.Results The LC50 of JYP was 1192 µg/mL.No significant toxicity was observed at concentrations of 50-400 µg/mL.However,higher concentrations caused embryonic developmental abnormalities and cardiotoxicity.Treatment with JYP significantly inhibited neutrophil infiltration(P<0.01),modulated NO levels(P<0.01),and downregulated the mRNA expression of NLRP3,Caspase-1,IL-1β,TNF-α,and IL-4(P<0.01).Conclusion The LC50 of JYP was 1192 µg/mL,with an embryo-safe concentration of≤400 µg/mL.Higher concentrations may induce developmental toxicity and cardiotoxicity.The anti-inflammatory effects of JYP likely involve multi-target mechanisms:inhibiting the NLRP3 inflammasome pathway(NLRP3,Caspase-1),reducing pro-inflammatory mediators(TNF-α,IL-1β),and modulating the Th2-type immune response(IL-4).This synergistic action suggests a bidirectional immunomodulatory property,mitigating excessive inflammation while promoting immune homeostasis.