线粒体复合物Ⅰ亚基NDUFS4在东亚三角涡虫再生中的功能解析OA

This study aimed to elucidate the role of NADH dehydrogenase(ubiquinone)iron-sulfur protein 4(NDUFS4),a core structural subunit of mitochondrial complex Ⅰ(CⅠ),in maintenance of adult stem cell function and neural regeneration in planarians.The ndufs4 gene was cloned from Dugesia sp.,and sequence together with structural prediction analyses revealed that the encoded protein is highly conserved in canonical domains and key amino acid residues.To assess its function during regeneration,RNA interference was used to knockdown the ndufs4 expression.Blastema growth rates in regenerating planarians were quantified,and in situ hybridization and immunohistochemistry were performed to assess adult stem cell(Neoblast)prolifera-tion and differentiation,as well as neural regeneration.The results showed that ndufs4 knockdown signifi-cantly reduced regeneration speed and severely impaired neural regeneration.Concurrently,the expression levels of stem cell marker genes were markedly decreased,and stem cell differentiation was disrupted.To-gether,these findings indicate that NDUFS4 is an essential for maintaining the adult stem cell population and promoting neural regeneration in planarians,highlighting its critical regulatory role in biological regeneration.