几种海南适生丛生竹扦插生根配方筛选OA

To accelerate the breeding of excellent sympodial bamboo in Hainan province and screen optimal rooting formulas for cutting propagation of excellent bamboo species,this study compares and analyzes the nutrients and enzyme activities in cuttings of various bamboo species across different months,aiming to identify the suitable period for cutting propagation.An orthogonal experiment is designed to study the effects of types,concentrations,treatment duration,and substrate of plant growth regulator on the rooting of cuttings of Guadua angustifolia,Dendrocalamus brandisii,Bambusa grandis and B.beecheyana.The results show that in July,the soluble sugar content of all bamboo species is the highest,and there is a high content of chlorophyll and total flavonoids and favorable IAAO activity,making the month a suitable period for cutting rooting of the four bamboo species.B.grandis and B.beecheyana show the best rooting results,with rooting rates reaching 57.73％and 41.36％,respectively.The optimal rooting formula for G.angustifolia is soaking in 200 mg/L NAA for 0.5 min with a substrate of peat:perlite at 2:1;for B.beecheyana,the optimal formula is soaking in 100 mg/L IAA for 1 minute with a substrate of peat:perlite:vermiculite at 2:1:1;for G.angustifolia,the optimal formula is soaking in 100 mg/L NAA for 1 minute with a substrate of peat:perlite at 2:1;and for D.brandisii,the optimal formula is soaking in 400 mg/L IAA for 2 minutes with a substrate of peat:vermiculite at 2:1.In the future,the research on the cuttage rooting can be further conducted for various bamboo species by considering factors such as bamboo species,branch age,collection parts,and cutting specifications.This will help establish a comprehensive propagation technology for major economically important bamboo species in Hainan province.