油茶ANXs基因的序列分析及其表达特征研究OA

Plant annexin is a conserved calcium-dependent phospholipid binding protein involved in plant growth,develop-ment and stress resistance.The sequence characteristics and expression patterns of the ANX gene family in C.oleifera remain unclear.For this reason,the present study aims to investigate the expression regulatory mechanisms of this gene family in the stress resistance responses of C.oleifera.Three ANX genes were identified from the whole genome of C.oleifera,and the physical and chemical characteristics,evolutionary relationship,gene structure,conserved sequence,chromosome location,expression level in different tissues and the content of annexin under drought stress were analyzed in the study.Phylogenetic analysis showed that 15 different families were clustered into 5 groups,among which CsANX and CoANX1/2 from Camellia were clustered into a large branch with similar motif patterns.Many cis-elements involved in light response and plant hormone response were identified in the promoter region of CoANX.Chromosome localization showed that the three CoANX genes were distributed on three chromosomes(A_chr 09/13/14)of C.oleifera.The expression pattern analysis showed that CoANX showed tissue-specific patterns in different growth stages and tissues.In addition,stress analysis showed that CoANX3 showed a high expression trend under drought conditions,while the expression of CoANX3 in the treatment group sprayed with brassinolide decreased.Under phosphorus and aluminum stress,the expression of ANX in C.oleifera increased significantly.The above results indicate that CoANX may play a key role in the stress resistance of C.oleifera.This study would lay a foundation for elucidating the molecular mechanism of ANXs in regulating the biological processes of plant growth and de-velopment.