基于网络药理学与体内实验研究胡芦巴对糖尿病肾病的抗炎和抗氧化作用OA

Objective To explore the anti-inflammatory and antioxidant effects of fenugreek on diabetes nephropathy through network pharmacological analysis and in vivo experimental verification.Methods TCMSP,SwissTarget and UniProt databases were used to screen,supplement and correct the active components of fenugreek and related targets.GeneCard,DisGNet,DigSee,TTD,OMIM,and DrugBank databases were used to obtain relevant targets for diabetic nephropathy;PPI network construction with STRING 11.0;GO and KEGG enrichment analysis were performed using Cytoscape 3.7.1 software;Blood glucose and blood lipid indexes of each group were detected by in vivo experiment,including FBG,TG and TC levels.Renal function indicators:BUN,SCR and 24h-mALB levels;Oxidative stress related indexes:SOD,MDA and CAT levels;Levels of inflammatory cytokines CCL2,IL-6 and ICAM-1.Results 14 active ingredients and 171 intersecting targets of"fenugreek-diabetic nephropathy"in fenugreek were obtained,and 2 core modules related to the regulation of inflammatory response were screened,and GO and KEGG enrichment analysis showed that the AGE-RAGE signaling pathway may be the key pathway for the treatment of diabetic nephropathy,and the AGE-RAGE signaling pathway was related to inflammatory factors and oxidative stress responses.Conclusion Fenugreek can regulate the AGE-RAGE signaling pathway through anti-inflammatory and antioxidative stress and play a therapeutic role in diabetic nephropathy.