Tiam1差异表达对卵巢癌预后及其生物学功能的影响OA

Objective To investigate the expression levels and clinical value of Tiam1 in ovarian cancer,and to unveil its effects on the biological processes of ovarian cancer cells.Methods Databases such as cBioPortal and SangerBox were used to analyze the mRNA alterations and expression levels of Tiam1 across various tumor types.Bio-informatics analyses were conducted to investigate Tiam1 expression in ovarian cancer tissues and its correlation with patient prognosis.The Linkedomics database was utilized to identify Tiam1-regulated downstream genes and associ-ated signaling pathways in ovarian cancer.Immunohistochemical SP staining was performed to detect the protein ex-pression of Tiam1 in ovarian tissues.Stable ovarian cancer cell lines with differential Tiam1 expression were con-structed to assess the effects of Tiam1 on cell proliferation,migration,and epithelial-mesenchymal transition(EMT)process.Results Multi-database analyses revealed that Tiam1 mRNA expression levels were significantly higher in various tumor tissues including ovarian cancer than in normal tissues(P<0.05).Additionally,high Tiam1 expression was closely associated with the increased malignancy,poor prognosis,and altered chemotherapeutic drug sensitivity in ovarian cancer patients(P<0.05).Immunohistochemical staining further confirmed that Tiam1 protein showed posi-tive expression in ovarian cancer tissues and negative expression in benign ovarian lesions(P<0.05).Both enrich-ment analysis based on the Linkedomics database and in vitro cell function assays consistently demonstrated that Tiam1 expression was significantly associated with enhanced cell migration and promoted the EMT process in ovarian cancer cells.Conclusion High Tiam1 expression predicts poor prognosis and increased sensitivity to chemotherapeutic drugs.Additionally,Tiam1 promotes cancer cell proliferation,migration,and EMT process in ovarian cancer cells,making it a promising novel biomarker for targeted therapy in ovarian cancer patients.