子宫内膜异位症中CMTM6、PD-L1的表达及意义OA

Objecitve To investigate the expression of CMTM6 and PD-L1 in endometriosis(EM)and their sig-nificance.Methods Ovarian ectopic endometrial tissues obtained by laparoscopic resection and eutopic endometrial tissues collected by curettage were obtained from 50 patients with EM(experimental group).Eutopic endometrial tis-sues from 30 non-EM patients(control group)were collected by curettage.The expression levels of CMTM6 and PD-L1 were detected by immunohistochemical method.Clinicopathological data of the experimental group,including age,body mass index(BMI),lesion size,serum CA-125 concentration,and rAFS score,were collected.The correlations between the expression of CMTM6 and PD-L1 and the above pathological parameters were analyzed by Spearman corre-lation coefficient.Human endometriotic epithelial cell line 12Z was cultured,western blot and co-immunoprecipitation were used to detect the expression levels and interaction of CMTM6 and PD-L1.Results CMTM6 and PD-L1 were highly expressed in EM patients,and their expression levels in ectopic and eutopic endome-tria of EM patients were higher than those in eutopic endometria of non-EM patients(P<0.05).The positive expres-sions of CMTM6 and PD-L1 were co-localizeed in the plasma membrane of epithelial cells in both ectopic and eutopic endometria,and their expression levels showed a positive correlation(P<0.05).Patient age,BMI,lesion size,serum CA-125 concentration,and rAFS score were positively correlated with CMTM6 and PD-L1 expression(P<0.01).CMTM6 and PD-L1 proteins were highly expressed in 12Z cells,and they could bind to each other.Conclu-sion CMTM6 and PD-L1 are highly expressed in EM patients,and the expression levels of CMTM6 and PD-L1 are correlated with the severity of EM lesions.CMTM6 and PD-L1 can interact in EM to jointly participate in the occur-rence and development of EM.CMTM6 and PD-L1 may be potential biological markers of EM,providing new targets for clinical diagnosis and treatment of EM.